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Table 3 Nucleotide sequences of used primers

From: Thermoanaerobacterium thermosaccharolyticum β-glucosidase: a glucose-tolerant enzyme with high specific activity for cellobiose

Primer

Nucleotide sequence

bgl-1

CCCCATATGTCGGACTTTAACAAGGAC

bgl-2

CCCCTCGAGAATGGTCCTAGTGGAAATAAG

bgl-3

TTTGGCGTGGCGACCGCGAGCTATCAGGTGGAAGG TGCTTACAATGAGGA

bgl-4

CAGAAAATCTTTGTTAAAATCGCTCATATGTATATCT CCTTCTTAAAG

  1. The boldface italic nucleotides represented mutations for optimizing codons.
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Biotechnology for Biofuels and Bioproducts

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