Effect of exposure to air on hydrolysis in spent medium in the presence of EDTA. Glucose produced (g/L) after 46 hours from the hydrolysis of 50 g/L Avicel in spent medium with 4 g/L ethanol under aerobic and anaerobic conditions, with and without 5 mM EDTA. Glucose production under anaerobic conditions shown in blue, under an aerobic headspace in red. Thus, the presence of EDTA prevented the increase in hydrolysis by exposure to air. Error bars indicate standard error of the mean for duplicates.