Introducing a single secondary alcohol dehydrogenase into butanol-tolerant Clostridium acetobutylicum Rh8 switches ABE fermentation to high level IBE fermentation

Table 2 Strains and plasmids used in this study

Strains and plasmids	Relevant charactristics	Soure or reference
Strains
C. acetobutylicum DSM 1731	Wild type strain	DSMZ
C. acetobutylicum Rh8	The mutant of C. acetobutylicum DSM 1731	[15]
C. beijierinckii NRRL B593	Contains sADH gene, wild type	NRRL
C. acetobutylicum	sADH expressing strain, harboring psADH	This study
Rh8(psADH)	Control strain harboring pIMP1	This study
C. acetobutylicum
Rh8(pIMP1)
E. coli JM109	recA1 mcrB + hsdR17	Lab storage
E. coli ER2275(pAN1)	Used for plasmid methylation before transformed into C. acetobutylicum	[26]
Plasmids
pAN1	ɸ3tI, p15a ori, Cm^r	[26]
pIMP1	The control plasmid, MLS^r, Amp^r, shuttle vector of E. coli-C. acetobutylicum	[26]
pITF	MLS^r, Amp^r, pIMP1 derivative for fdh expression under thl promoter	[27]
psADH	Used for sADH expression with thl promoter	This study

Abbreviations: Amp^r, ampicillin resistance; Cm^r, chloramphenicol resistance; MLS^r, macrolide, lincosamide, and streptogramin B resistance; ɸ3tI, ɸ3TI methyltransferase gene of Bacillus subtilis phage ɸ3TI; thl, the promoter of thiolase gene in C. acetobutylicum; DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany. NRRL, Agricultural Research Service Culture Collection, United State.

ISSN: 2731-3654