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Table 2 Strains and plasmids used in this study

From: Introducing a single secondary alcohol dehydrogenase into butanol-tolerant Clostridium acetobutylicum Rh8 switches ABE fermentation to high level IBE fermentation

Strains and plasmids Relevant charactristics Soure or reference
C. acetobutylicum DSM 1731 Wild type strain DSMZ
C. acetobutylicum Rh8 The mutant of C. acetobutylicum DSM 1731 [15]
C. beijierinckii NRRL B593 Contains sADH gene, wild type NRRL
C. acetobutylicum sADH expressing strain, harboring psADH This study
Rh8(psADH) Control strain harboring pIMP1 This study
C. acetobutylicum  
E. coli JM109 recA1 mcrB + hsdR17 Lab storage
E. coli ER2275(pAN1) Used for plasmid methylation before transformed into C. acetobutylicum [26]
pAN1 ɸ3tI, p15a ori, Cmr [26]
pIMP1 The control plasmid, MLSr, Ampr, shuttle vector of E. coli-C. acetobutylicum [26]
pITF MLSr, Ampr, pIMP1 derivative for fdh expression under thl promoter [27]
psADH Used for sADH expression with thl promoter This study
  1. Abbreviations: Ampr, ampicillin resistance; Cmr, chloramphenicol resistance; MLSr, macrolide, lincosamide, and streptogramin B resistance; ɸ3tI, ɸ3TI methyltransferase gene of Bacillus subtilis phage ɸ3TI; thl, the promoter of thiolase gene in C. acetobutylicum; DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany. NRRL, Agricultural Research Service Culture Collection, United State.