Relative expression of desaturase genes. (A) Fatty acid desaturase (FAD). (B) Fatty acid desaturase 5 (FAD5). (C) Fatty acid desaturase 6 (FAD6). (D) Acyl-ACP desaturase (AAD). (E) δ-12-acyl-lipid desaturase (DALD). (F) δ-7-C-5 sterol desaturase (D7SD). (G) δ-9-stearoyl-acyl carrier protein desaturase (D9SD). (H) δ-12 fatty acid conjugase (D12FAC). (I) Fatty acid desaturase 2 (FAD2). (J) Sterol desaturase (SD). Relative expression of desaturase genes in endosperm was detected at different developmental stages. The gene transcripts were measured by qRT-PCR. Results are shown as the relative expression of genes at different developmental stages by comparing to itself at the highest expression, which was set as “1”. The experiments were performed in triplicate and the data are presented as means ± SD.