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Table 2 Identified cellulose-degrading proteins and spectrum counts on 1, 3, 7, and 14 days

From: A time course analysis of the extracellular proteome of Aspergillus nidulans growing on sorghum stover

    

Spectrum countc

 

Accession numbera

GH familya

Identified proteinsa

MW (kDa)b

Day 1

Day 3

Day 7

Day 14

SignalPd

AN9183

GH1

Beta −1,4-glucosidase

66

11

14

22

14

Y

AN2227

GH3

Beta-1,4-glucosidase

92

9

0

0

0

Nf

AN2828

GH3

Beta-1,4-glucosidase

78

33

144

131

156

Y

AN4102

GH3

Beta glucosidase

92

78

222

204

215

Y

AN5976

GH3

Beta glucosidase

89

53

105

22

0

Y

AN7396

GH3

Beta glucosidase

84

0

116

107

59

Y

AN1804

GH3

Beta-1,4-glucosidase

68

4

4

49

31

Y

AN10482

GH3

Beta-1,4-glucosidase

94

0

9

21

10

Y

AN1285

GH5

Beta-1,4-endoglucanase

36

21

49

38

42

Y

AN8068

GH5

Putative endoglucanase

63

0

20

46

28

Y

AN9166

GH5

Cellulase family protein

45

0

9

0

5

Y

AN1273

GH6

Cellobiohydrolase

41

12

37

23

39

Y

AN5282

GH6

Cellobiohydrolase

47

0

15

49

54

Y

AN0494

GH7

Cellobiohydrolase

56

15

33

58

80

Y

AN5176

GH7

Cellobiohydrolase

48

63

142

195

234

Y

AN3418

GH7

Beta-1,4-endoglucanase

46

65

82

76

88

Y

AN2664

GH43

Beta-glucanase, putative

55

0

0

0

7

Y

AN3046

GH61

Endoglucanase, putative

32

44

0

0

0

Y

AN3860

GH61

Endoglucanase IV precursor

26

5

0

14

17

Y

AN10419

GH61

Beta-1,4-endoglucanase

29

0

10

10

16

Y

AN6428

GH61

Endoglucanase 4

24

2

0

5

7

Y

  1. aAccession numbers along with protein information and glycosyl hydrolase (GH) family information was obtained from Pedant [23].
  2. bHypothetical molecular weight of the proteins.
  3. cQuantifying changes in protein abundance between samples from different time points was done using the spectral count method, yielding a semiquantitative analysis.
  4. dSignalP was used to predict secretion signals [23, 24].
  5. eSignalP as reported at [25].
  6. fNot found by SignalP (N-terminal may be incorrectly annotated , a novel signal peptide may be present, or the protein is normally intracellular but was released by autolysis).