A time course analysis of the extracellular proteome of Aspergillus nidulans growing on sorghum stover

Table 5 Identified fungal cell wall degradation/remodeling proteins and spectrum counts on 1, 3, 7, and 14 days

				Spectrum count^c
Accession number^a	GH family^a	Identified proteins^a	MW (kDa)^b	Day 1	Day 3	Day 7	Day 14	SignalP^d
AN0933	GH16	Extracellular cell wall glucanase	42	18	35	11	7	Y
AN0245	GH16	Beta-1,3(4)-endoglucanase, putative	37	0	33	15	29	Y
AN6620	GH16	Beta-1,3(4)-endoglucanase, putative	42	4	0	0	0	Y
AN6819	GH16	Endo-1,3 (4)-glucanase	32	9	7	8	7	Y
AN7950	GH17	Cell wall beta-1,3-endoglucanase	47	17	32	32	26	Y
AN4871	GH18	Protein similar to class V chitinase A	44	5	224	277	317	N^f
AN8241	GH18	Class III Chi A chitinase	97	0	5	2	0	Y
AN1502	GH20	Protein similar to N-acetylglucosaminidase	68	11	101	124	176	Y
AN0779	GH55	Putative beta-1,3-exoglucanase	84	0	19	19	15	Y
AN4825	GH55	Glucan 1,3-beta glucosidase precursor	97	0	102	108	135	Y
AN9042	GH71	Putative alpha 1,3- glucanase	69	0	51	55	60	Y
AN7657	GH72	1,3-beta-glucanosyltransferase	49	14	37	0	4	Y
AN0472	GH81	Putative beta-1,3-endoglucanase	98	0	102	99	146	Y
AN9339		Catalase B precursor	79	58	111	109	108	Y
AN4390		GPI-anchored cell wall organization protein Ecm33	41	4	7	-	-	Y
AN2385		GPI anchored beta-1,3(4)-endoglucanase, putative	65	3	-	-	-	Y

^aAccession numbers along with protein information and glycosyl hydrolase (GH) family information was obtained from Pedant [23].
^bHypothetical molecular weight of the proteins.
^cQuantifying changes in protein abundance between samples from different time points was done using the spectral count method, yielding a semiquantitative analysis.
^dSignalP was used to predict secretion signals [23, 24].
^eSignalP as reported at [25].
^fNot found by SignalP (N-terminal may be incorrectly annotated , a novel signal peptide may be present, or the protein is normally intracellular but was released by autolysis).

ISSN: 2731-3654