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Table 4 List of primers used in this study

From: Functional expression and characterization of five wax ester synthases in Saccharomyces cerevisiae and their utility for biodiesel production

Gene product

Primer sequence 5'→3'

 

forward

reverse

Wax ester synthase from A. baylyi ADP1 [GenBank: AF529086]

CGGGATCCCGCTCGAGATGCGTCCATT

GGGGTACCCCAAGCTTGGGTTAGTTTGCAG

Wax ester synthase from M. hydrocarbonoclasticus DSM 8798 (EF219377)

CGGGATCCCGCTCGAGATGAAGAGATTAGG

GGGGTACCCCAAGCTTGGGTTACTTTCTAGTACG

Wax ester synthase from R. opacus PD630 (GQ923886)

CGGGATCCCGCTCGAGTTGACCGACGTGATTAC

GGGGTACCCCAAGCTTGGGTTAGCTAGCCACCACC

Wax ester synthase from Mus musculus C57BL/6 (AY611032)

CGGGATCCCGCTCGAGATGTTCTGGCCAACC

GGGGTACCCCAAGCTTGGGTTAAACAATGACCAAC

Wax ester synthase from P. arcticus 273-4 (YP_263530)

CGGGATCCCGCTCGAGATGAGATTACTGACCGCTGT

GGGGTACCCCAAGCTTGGGTTAAGGGGCCAACT

Acetyl-CoA carboxylase [GenBank: NM_001183193] from S. cerevisiae

ATTTGCGGCCGCTTTAGTTTCTACCATGAGCGAAG

GGCGAGCTCGCAAGGTTTATTTCAAAGTCTTC

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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