Microbial β-glucosidases from cow rumen metagenome enhance the saccharification of lignocellulose in combination with commercial cellulase cocktail

Table 1 Kinetic parameters of the purified β-glucosidases

Enzyme	Substrate	K_m(mM)^a	k_cat(s^-1)^a	k_cat/K_m(s^-1 M^-1)^a
SRF2g14	p NPβGlu	0.034 ±0.003	0.37 ± 1.34	10.76 × 10³
	p NPβC	0.48 ±0.054	0.19 ± 0.36	4.16 × 10²
	Cellobiose	8.02 ±0.939	0.38 ± 0.55	47.64
SRF2g18	p NPβGlu	1.73 ± 0.004	0.27 ± 0.32	1.59 × 10²
	p NPβC	0.48 ±0.072	0.11 ± 0.22	2.32 × 10²
	Cellobiose	25.51 ±2.46	0.09 ± 0.31	3.35
LAB20g4	p NPβGlu	0.03 ±0.003	0.88 ± 4.05	29.49 × 10³
	p NPβC	1.47 ± 0.13	0.12 ± 0.20	82.73
	Cellobiose	7.80 ± 0.67	0.22 ± 0.35	28.75
LAB25g2	p NPβGlu	0.45 ± 0.06	0.41 ± 0.68	9.20 × 10²
	p NPβC	0.39 ± 0.03	0.17 ± 0.36	4.38 × 10²
	Cellobiose	4.88 ± 0.59	0.10 ± 0.23	20.84

^aParameters were determined at 40°C and pH 5.6 (50 mM sodium acetate) using substrate and enzyme concentrations as described in Methods.

ISSN: 2731-3654