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Table 2 Primers pairs used for q-PCR analysis with target gene information

From: Transcriptome profiling of Zymomonas mobilis under ethanol stress

Primary Locus

Gene

Function

Forward primer (5′to 3′)

Reverse primer (5′to 3′)

Product size (bp)

aArray

bqPCR

ZMO0216

 

peptidase M23

GACATCACTGGCTTCTAA

GCTGGTTCAAGACGATAT

104

1.1

2.6

ZMO0265

 

hypothetical protein

TAAACAGCAGATGACCTT

ATATTGGACCGATTGGAA

100

2.3

2.3

ZMO0375

 

levansucrase

TTATGCGGATAGTGAAGG

ACGGAAATTCCAGAGATTA

115

3.0

7.2

ZMO0546

 

sulphate transporter

TGTCCTGACTCATAATCT

CGCTTATTCTCTTCATCA

120

3

4.8

ZMO0557

 

hypothetical protein

AGATTATCAGGACTGGAA

TAACATTATCAGCATCGT

113

1.5

3.1

ZMO1417

 

DEAD/DEAH box helicase domain-containing protein

TTATTGCCAATGACGAAC

TTTTCCATGACAAAGTTTTC

100

1.5

3.1

ZMO1425

 

thiamine monophosphate synthase

TCATTATCGCTTGCCCTTCA

GAGCCGAATCAGCCAGAA

101

1.1

4.9

ZMO1802

 

hypothetical protein

TGCTTATGCAGTGTTTGG

TCAGGAAGGTGTAGAGAC

94

1.1

3.5

ZMO1804

 

amino acid permease-associated domain-containing protein

TTTATGGATTTGATACTGTC

CGCTACACCAATATAGAT

119

1.4

3.71

ZZM4_0013

 

P2 GpU family protein

GTCACATCCATAGTAGAA

TTATTGTATTGTCGTCATC

106

1.7

4.3

ZZM4_0036

 

protein of unknown function DUF264

CCAGAATAGTGAAGAAGG

ATCAAGACCTCTAAGTTG

109

1.4

1.13

pzmob1_p05

 

hypothetical protein

TTCCAATCGGTTCAATTAGT

CAGCCATAGTATCGGTAAG

100

2.11

4.86

pzmob1_p07

 

hypothetical protein

ATGCTGCTTGGTTTGTTA

GTCATCACAATAGGTAGTCT

107

2.66

4.09

ZMO1063

pspA

phage shock protein A

GCCTTATCAGCGATTTATC

GCTTCATTCAACTTATTCTG

100

1.1

2.5

ZMO1064

pspB

phage shock protein B

TTAGTCTGCCTTATTCTG

CTCATAAAGCTCTTCAATC

120

1.3

2.0

ZMO1065

pspC

phage shock protein C

AAACCGTTTTCGTGATAT

CGCAAATTATCTATTTCCTT

96

1.2

3.0

ZMO0057

 

phage protein

GATAAAGCGACATTAAAGG

TTCATCACCCAGTATTTC

111

−1.2

−3.21

ZMO0062

 

aldo/keto reductase

CAACCCGAATATAATCTTTA

AAGCCAGACTGTAATAAG

103

−1.52

−4.7

ZMO1851

 

flavodoxin FldA

AAATTGACTGGGAGGATA

GACGAAAGAATCTGGATAA

110

−1.93

−4.7

Endogenous control

     

ZMOr009

rrsA

16s RNA

TCAACTATAGACCAGTAAGT

AGAACATAGAAGAGGTAAGT

101

  
  1. aArray: the log2 based microarray ratio of the gene expression (ethanol stress/normal).
  2. bqPCR: the log2 based qPCR ratio of the gene expression (ethanol stress/normal).
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