Changes in differential weight distributions at different times during enzymatic hydrolysis. (a, c, e) untreated and (b, d, f) regenerated cellulose using (a, b) Avicel, (c, d) α - cellulose (e, f) Sigmacell. Areas under the curves were adjusted to represent weight loss during the hydrolysis. Enzymatic hydrolysis performed at 45°C using 0.25 g/L desalted Celluclast® in 0.1 M NaAc buffer pH 4.8. Eluent DMF/10% (v/v) EMIM Ac, flow rate 0.5 mL/min.