Figure 2
From: Derivatization-free gel permeation chromatography elucidates enzymatic cellulose hydrolysis
Changes in differential weight distributions at different times during enzymatic hydrolysis. (a, c, e) untreated and (b, d, f) regenerated cellulose using (a, b) Avicel, (c, d) α - cellulose (e, f) Sigmacell. Areas under the curves were adjusted to represent weight loss during the hydrolysis. Enzymatic hydrolysis performed at 45°C using 0.25 g/L desalted Celluclast® in 0.1 M NaAc buffer pH 4.8. Eluent DMF/10% (v/v) EMIM Ac, flow rate 0.5 mL/min.