Figure 6

Simultaneous saccharification and fermentation (SSF) of ammonia pretreated sugarcane bagasse using S. stipitis NRRL Y-7124 at 30°C and pH 5.0. Pre-hydrolysis of ammonia pretreated SB was carried out for 24 h to initiate the enzymatic saccharification. After, 24 h of pre-hydrolysis, fermentation medium ingredients and inoculum was added to start SSF for ethanol production.