SS sum of square, df degree of freedom, MS mean square, F Statistical test (explained variance/unexplained variance), p probability. Effect of all three process variables (Temperature, pretreatment time and ammonium hydroxide concentration) and their interaction on sugars recovery. Temperature and pretreatment time at individual level showed maximum sum of squares (SS) compared to other variables. Pretreatment time showed major influence (p value, 0.05382) followed by temperature (p value, 0.061013). Ammonia concentration at individual level did not present major influence on sugars recovery.