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Figure 6 | Biotechnology for Biofuels

Figure 6

From: Microevolution from shock to adaptation revealed strategies improving ethanol tolerance and production in Thermoanaerobacter

Figure 6

Genetic approaches that improved both ethanol production and tolerance. (A-B) Transcriptional programs of nine adhs and six Ï­ factors from ethanol shock to tolerance. (C-D) Growth curves of Thermoanaerobacter sp. X514 wild-type strain and wild-type strains that carried on the pIKM1 plasmid: a vector-only control, an adh locus (Teth5140145-0146) or a Ï­24 cluster (Teth5141847-1848) under 0% (C) and 1% (D) exogenous ethanol. Strains were grown at 45oC in QRCM medium. (E) Ethanol production of these engineering strains (Xvector, X adhE and XÏ­24) at stationary phase, which were grown at 45oC in QRCM medium.

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