Figure 6

Percent lignin removal from Populus trichocarpa x P. deltoides wood samples and cellulolytic enzyme lignin (CEL) after batch (0 mL/min, diagonal hatching) and flowthrough (20 mL/min, solid) pretreatments at 140 and 180°C for 12 and 192 minutes. Percent lignin removal was calculated from the Klason lignin and total solids mass balance. See Additional file 1, Additional file 2, Additional file 3, and Additional file 4 for details of the calculations.