Transient expression and purification of LipA in Nicotiana benthamiana leaves. (A) Gel code blue stained SDS-PAGE analysis of protein purification from N. benthamiana leaves co-infiltrated with LipA and the silencing suppressor p19. Leaf discs were collected 4 days post infiltration (dpi). Lane 1: Extract flowthrough, Lane 2 to 4: Washes and Lane 5: eluted LipA. (B) Western-blot analysis of a gel loaded with 5 μg total soluble protein. Lane 1: LipA from plant extract, lane 2: wt plant extract, lane 3 to 5: C-myc standard (24, 72, and 216 ng respectively).