Figure 5

Effect of pH and temperature on LipA enzyme activity. (A) The influence of pH on activity was determined using 1 mM p NP-caprylate in 100 mM buffers (pH 4.0-5.0 acetate buffer, pH 6.0-9.0 phosphate buffer, pH 10.0-11.0 carbonate buffer) at 40°C for 30 min. (B) The influence of temperature was determined in a range from 20-90°C using 1 mM p NP caprylate in 100 mM phosphate buffer pH 7.0 for 30 min. The test was performed in triplicate and the average values were transformed in the relative activity (%).