Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment

Table 4 Diversity of GH families returned by JGI and Uniprot databases from the secretomes analyzed

Organism	Culture																Glycoside hydrolase families
I. lacteus	SSF		2	3*		5	6	7		10														35		43*				61			74					92*
I. lacteus	SmF		2	3		5								15								30	31	35		43*												92*			125
P. chrysosporium	SSF			3		5	6	7	8	10	11	12		15		17	18	20	25	27	28	30	31	35	37	43	47			61	71						88
P. ostreatus	SSF	1	2	3	4	5	6	7	8	10		12	13	15	16			20		27	28		31	35	37		47	51	55			72		76	78	79	88	92	105	115
Type of enzymes ¹ Cellulose degrading enzymes
Cellulases									X
Endo-1,4-β-glucanases				X		X		X				X			X													X					X
Cellobiohydrolases							X	X		X																							X
Exo-1,4-β-glucanases		X		X			X
β-Glucosidases		X		X																		X
Hemicellulose degrading enzymes
Xylanases/xyloglucanases						X				X	X	X				X										X							X	X
Endo-1,4-β-xylanases				X		X			X						X
Endo-1,3-β-xylanases										X																X
β-Xylosidases																						X				X
Arabinofuranosidases				X																						X		X
α- Glucuronidases					X																															X				X
β-Galactosidases		X	X																					X
β-Mannosidases		X	X			X
Starch degrading enzymes													X	X									X				X
Other GHs ²		X	X	X	X	X	X	X	X			X				X	X	X	X	X	X	X		X	X	X		X	X	X	X	X		X	X	X	X	X	X	X	X

Crosses (X) show the GH family to which the proteins identified in the different secretomes belong, according to http://www.cazy.org/Glycoside-Hydrolases.html.
(*) These GH families were not detected in I. lacteus’ spots, but were identified from EPP analyses.
(¹) Each functional group contains the most representative enzymes.
(²) This group contains activities different to those detailed in the Table.

ISSN: 2731-3654