Figure 6

Synthetic operons inserted into the C. acetobutylicum chromosome. Encoded genes are: thl, thiolase; ermB, erythromycin ribosomal methylase B, C. thermocellum derived cellulases cel9B and cel8A, mini-scaffoldin cipA3. All operons are preceded by the chromosomal thl promoter and the celllulosomal components by an additional synthetic thlOID promoter and followed by a transcriptional terminator (from Clostridium pasteurianum ferredoxin gene).