Figure 3

Growth and in vivo hydrogen production by strains expressing SH hydrogenase. Cultures, pregrown under the same conditions, were incubated at 37°C in anaerobic vials containing modified M9 glucose (+IPTG, Ni and Fe). Samples were taken periodically to measure OD (A) and hydrogen (B). FTGH2/pJWPH5 (-♦-); FTJWDC3/pJWPH5 (-■-); JWGH1/pJWPH5 (-△-); DJ1/pJWPH5 (-○-); DG2/ pJWPH5 (-□-); FTDPH10/ pJWPH5 (-●-).