Table 1 Plackett–Burman design for screening the variables, effect of each variable on 2,3-BD production, and statistical analysis of variables in the fermentation of glucose using strain 10-1-A

X ₁

2

4

2.061

0.5149

4

0.01 ^a

X ₂

5

10

2.335

0.5149

4.53

0.006 ^a

X ₃

2

4

2.321

0.5149

4.51

0.006 ^a

X ₄

2

4

1.051

0.5149

2.04

0.097

X ₅

1

2

−1.160

0.5149

−2.25

0.074

X ₆

0.25

0.5

−0.567

0.5149

−1.1

0.321