Table 3 Plasmids used in this study
From: Carbon dioxide fixation by Calvin-Cycle enzymes improves ethanol yield in yeast
Name | Relevant genotype | Source/reference |
|---|---|---|
pFL451 | AOX1p-prk (Spinach)-AOX1t (pHIL2-D2 HIS4 Amp centromeric) | Brandes et al.[9]. |
pCR®-Blunt II-TOPO | Bla | Life Technologies Europe BV |
pTEF424_TEF | TRP1 2ÎĽ bla | Mumberg et al.[28]. |
pSH47 | URA3 CEN6 ARS4 GAL1p-cre-CYC1tbla | GĂĽldener et al. [29]. |
pUD0E46 | TRP1 2ÎĽ GAL1 p-prk-CYC1tbla | This study. |
pPCR-Script | Bla | Life Technologies Europe BV. |
pGPD_426 | URA3 2ÎĽ bla | Mumberg et al.[28]. |
pRS416 | URA3 CEN6 ARS4 bla | Mumberg et al.[28]. |
pBTWW002 | URA3 2ÎĽ TDH3p-cbbM-CYC1tbla | This study. |
pUDC098 | URA3 CEN6 ARS4 TDH3 p -cbbM-CYC1 t bla | This study. |
pMK-RQ | nptII | Life Technologies Europe BV. |
pUD230 | PGI 1p-cbbQ2-TEF2tnptII | Life Technologies Europe BV. |
pUD231 | PGK1p-cbbO2-ADH1tnptII | Life Technologies Europe BV. |
pUD232 | TEF1p-groEL-ACT1tnptII | Life Technologies Europe BV. |
pUD233 | TPI1p-groES-PGI1tnptII | Life Technologies Europe BV. |
pUDC075 | URA3 CEN6 ARS4 TDH3p-cbbM-CYC1t;PGI1p-cbbQ2-TEF2t;PGK1p-cbbO2-ADH1t;TEF1p-groEL-ACT1t;TPI1p-groES-PGI1tbla | This study. |
pUDC099 | URA3 CEN6 ARS4 TDH3p-cbbM-CYC1t;PGI1p-cbbQ2-TEF2t;PGK1p-cbbO2-ADH1tbla | This study. |
pUDC100 | URA3 CEN6 ARS4 TDH3p-cbbM-CYC1t;TEF1p-groEL-ACT1t;TPI1p-groES-PGI1tbla | This study. |