Clostridium thermocellum transcriptomic profiles after exposure to furfural or heat stress

Wilson, Charlotte M; Yang, Shihui; Rodriguez, Miguel; Ma, Qin; Johnson, Courtney M; Dice, Lezlee; Xu, Ying; Brown, Steven D

doi:10.1186/1754-6834-6-131

Biotechnology for Biofuels and Bioproducts

Table 2 Subset of genes with the highest (n = 10) and lowest (n = 10) differential expression to each of the treatments compared to control fermentations

From: Clostridium thermocellum transcriptomic profiles after exposure to furfural or heat stress

Locus tag	Functional annotation	Ratio: control – stress^a				Venn code
		10 min	30 min	60 min	120 min
Furfural stress
Cthe_0211	Glycoside hydrolase family 16	−2.22	−3.56	−2.41	−2.87	D
Cthe_1643	Phage-associated protein	−2.12	−4.49	−2.41	−2.77	F
Cthe_1644	Hypothetical protein	−2.26	−4.07	−2.27	−2.79	F
Cthe_2531	Sulfate ABC transporter, periplasmic sulfate-binding protein	−1.87	−3.19	−3.58	−3.99	F
Cthe_2532	Sulfate ABC transporter, inner membrane subunit CysT	−1.90	−2.92	−3.07	−3.55	D
Cthe_2533	Sulfate ABC transporter, inner membrane subunit CysW	−1.77	−2.82	−3.29	−3.46	D
Cthe_2535	Adenylylsulfate reductase, thioredoxin dependent	−1.54	−2.86	−3.27	−3.45	D
Cthe_2962	Oligopeptide/dipeptide ABC transporter, ATPase subunit	−1.87	−3.04	−2.15	−2.83	F
Cthe_2963	Oligopeptide/dipeptide ABC transporter, ATPase subunit	−1.96	−3.33	−2.15	−2.59	F
Cthe_3125	Heat shock protein Hsp20	−2.60	−3.21	−2.49	−2.30	B
Cthe_0422	Redox-sensing transcriptional repressor rex	2.98	3.47	2.81	3.48	B
Cthe_0424	Aminoglycoside phosphotransferase	1.98	3.14	2.23	2.93	B
Cthe_0425	Hypothetical protein	2.49	4.07	3.23	3.84	D
Cthe_0426	Fe-S cluster domain protein	2.47	3.86	2.82	3.60	B
Cthe_0427	Stage II sporulation protein E	2.31	4.20	2.46	3.42	D
Cthe_0428	NADH dehydrogenase (ubiquinone) 24 kDa subunit	3.08	5.49	3.05	4.61	F
Cthe_0430	Hydrogenase, Fe-only	1.79	4.83	2.90	4.06	F
Cthe_0431	Hypothetical protein	2.24	5.64	3.15	4.74	D
Cthe_0943	Hypothetical protein	2.53	3.30	2.63	2.86	C
Cthe_0944	SMC domain protein	2.20	2.88	2.32	2.63	C
Heat stress
Cthe_1309	Radical SAM domain protein	0.14	−1.94	−2.37	−2.14	A
Cthe_1743	Protein of unknown function DUF955	−1.89	−2.77	−2.58	−2.72	D
Cthe_1746	Hypothetical protein	−0.33	−1.99	−2.98	−1.82	D
Cthe_1851	Protein of unknown function DUF1113	−0.30	−2.84	−2.29	−1.76	D
Cthe_2448	ABC-type transporter, integral membrane subunit	−0.40	−2.82	−2.19	−1.66	A
Cthe_2449	Phosphoglycerate mutase	−0.03	−2.44	−2.04	−1.84	A
Cthe_3054	Hypothetical protein	0.01	−2.75	−2.03	−2.33	B
Cthe_3112	Glycosidase related protein	−0.71	−2.61	−2.52	−1.71	B
Cthe_3116	Mannose-6-phosphate isomerase, class I	−0.13	−2.78	−2.84	−2.11	B
Cthe_3125	Heat shock protein Hsp20	−0.06	−4.07	−2.80	−2.74	B
Cthe_0323	Hypothetical protein	−0.44	1.72	2.06	1.49	B
Cthe_0539	ABC transporter related	−0.23	2.10	2.18	2.23	B
Cthe_0665	HflK protein	0.13	2.47	2.95	2.03	A
Cthe_0938	Regulatory protein DeoR	−0.20	2.22	2.53	1.96	D
Cthe_1390	Alpha/beta hydrolase fold	1.26	3.66	2.91	2.75	D
Cthe_1921	Transcriptional regulator PadR family protein	1.03	1.77	2.94	2.65	A
Cthe_1922	Hypothetical protein	0.56	1.41	2.64	2.43	A
Cthe_2266	Vacuolar H+transporting two-sector ATPase F subunit	−0.03	1.49	2.58	1.87	E
Cthe_2956	Hypothetical protein	0.54	1.86	1.96	1.61	B
Cthe_2957	Hypothetical protein	0.53	2.01	1.56	1.57	B

^aNegative values indicate greater gene expression in the treatment samples while positive values indicate lower gene expression in the treatment samples compared to the control fermentations. Boldface indicates the differential gene expression was statistically significant (p < 0.05).

Back to article page

ISSN: 2731-3654

Contact us

General enquiries: journalsubmissions@springernature.com