Figure 1
From: Strong cellulase inhibitors from the hydrothermal pretreatment of wheat straw
The LF is a strong inhibitor for Tr Cel7A on both 14C-BC and MUL substrates. (A) 14C-BC (0.25 mg ml-1) was incubated with the mixture of 0.25 μM Tr Cel7A, 0.025 μM EG (Tr Cel5A), and 0.1 μM N188 BG at 25°C in the presence of no LF (□), N188 BG-treated LF (♦), or sulfuric acid–treated LF (▲). (B) MUL (5 μM) was incubated with 10 nM Tr Cel7A at 35°C in the presence of the original LF (◊) or the N188 BG-treated LF (♦).