Figure 3
The effect of cyanocobalamin concentration on 1-propanol production in strain WT-adhE2(CA)3. 1-Propanol production is dependent on the exogenous supplementation of cyanocobalamin and saturation occurs at concentrations above 0.2 μM. Strains were cultivated anaerobically in reduced M9 minimal media with 20 g/L of glucose at 37°C for 72 h. All experiments were performed in triplicate.