Figure 5
Single dimension hydrogen NMR spectra scanned at 600 MHz from samples of E. coli supernatant from strain WT-adhE2(CA)3. Strain was cultivated anaerobically in reduced M9 minimal media with 20 g/L of glucose at 37°C for 72 h. Culture samples were then centrifuged for 3 min at 13,000 × g to recover the supernatant fraction for analysis. A) The 25 OD600 spectrum profiled for metabolites using Chenomx Suite 7.5. B) Zoomed in panels from part A, identifying the three peak clusters of 1-propanol and major end-product metabolites. From left to right the panels show: i. lactate, glucose and ethanol peaks, ii. convolution of glycine spectra with that of the first 1-propanol peak cluster, iii. acetate, iv. the unobscured second peak cluster of 1-propanol, v. propionate, vi. the third peak cluster of 1-propanol. C) Zoomed in panels from part B of the three 1-propanol peak clusters from pure solution standard and supernatant of WT-adhE2(CA)3 grown at 25 OD600.