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Table 2 Comparison of 1-propanol production titers and other metabolites (mg/L) by expression of several ADHs in E. coli strain BW25141, transformed with appropriate plasmids

From: Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli

Strain

Metabolite titers (mg/L)

Succinate

Lactate

Acetate

Propionate

Ethanol

1-Propanol

Control

      

BW25141

307 ± 36

128 ± 11

4436 ± 250

—

3021 ± 156

—

Experimental

      

WT-adhE2(CA)3

231 ± 11

1877 ± 303

2653 ± 55

51 ± 14

2774 ± 297

103 ± 16

WT-adhP(EC)

239 ± 57

2986 ± 498

2545 ± 89

100 ± 18

3192 ± 80

84 ± 7

WT-yqhD(EC)

Trace

3322 ± 920

3818 ± 826

29 ± 67

3469 ± 538

69 ± 10

WT-adhEMUT(EC)

Trace

3762 ± 393

2164 ± 64

Trace

4016 ± 83

74 ± 6

WT-adhE1(CA)

Trace

411 ± 120

4247 ± 198

71 ± 10

4397 ± 403

76 ± 11

WT-bdhB(CA)

150 ± 131

2139 ± 474

2329 ± 21

67 ± 22

3455 ± 169

109 ± 6

  1. Cultures were suspended in reduced M9 minimal media and induced at an O.D600 of 15. Strains were cultivated anaerobically at 37°C for 72 h. Glucose (20 g/L) was used as the sole carbon source and all experiments were performed in triplicate.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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