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Table 1 Ethanol concentration and yield at different feed methods using fed-batch S-SSF

From: Tween 40 pretreatment of unwashed water-insoluble solids of reed straw and corn stover pretreated with liquid hot water to obtain high concentrations of bioethanol

Trial number Dry weight (g) of WIS fed at different timesb Volume of fermentation broth (mL) after feedinga Reed straw Corn stover
  At 6 hours of pre-hydrolysis At 16 hours of pre-hydrolysis At 6 hours of fermentation Reed straw Corn stover Ethanol concentration (g/L) Ethanol yield (%) Ethanol yield (g/kg feedstock) Ethanol concentration (g/L) Ethanol yield (%) Ethanol yield (g/kg feedstock)
1 1 - - 11.44 11.60 41.21 ± 1.42 74.4 248 39.20 ± 2.84 70.3 230
2 1 0.5 - 12.16 12.40 46.23 ± 2.84 71.0 237 51.26 ± 1.42 78.6 257
3 1 1 - 12.88 13.20 41.21 ± 1.42 55.9 186 52.26 ± 2.84 71.1 233
4 1 0.5 0.5 12.88 13.20 53.27 ±1.42 72.3 241 47.24 ± 1.42 64.3 211
5 1 1 0.5 13.60 14.00 56.28 ± 0 69.1 197 52.26 ± 0 64.6 212
  1. aCalculated value based on water content of fed WIS. The moisture of fed WIS was 59.02% for reed straw and 61.60% for corn stover; bweight of initial WIS was 1 g in the S-SSF. The Tween 40 pretreatment conditions were as follows: Tween 40 concentration, 1.5%; ratio of WIS-to-Tween 40, 1:10 (w/v); pretreatment temperature, 25°C; and pretreatment time, 1 hour. The fed-batch S-SSF conditions were as follows: cellulase loading, 25 FPU/g oven-dried WIS; pre-hydrolysis temperature, 50°C; pre-hydrolysis time, 18 hours; fermentation temperature, 36°C; and fermentation time, 72 hours. S-SSF, semi-simultaneous saccharification and fermentation.