Results of method control experiments using corn stover control material. Measured glucose and xylose release from (a) pretreatment assay, (b) enzymatic hydrolysis assay, and (c) combined pretreatment (PT) + enzymatic hydrolysis (EH) assay. As expected, the majority of xylose was released during pretreatment whereas the majority of glucose was released during enzymatic hydrolysis. The variability of the glucose release (SD = 2.1%) was greater than the variability of the xylose release (SD = 0.7%). The variability of these replicates limits the discriminating power of the assay.