Saccharification efficiency of WT, pme3 , PMEI, qua2-1, and PG leaves. Saccharification efficiency was measured after 24 hours of treatment with 0.5% Celluclast. Bars represent average of saccharification efficiency ± SE (n ≥4). Different letters indicate statistically significant differences, according to ANOVA followed by Tukey’s test (P <0.05). The experiments were repeated at least three times with similar results. PG, polygalacturonase; pme3, pectin methylesterase 3; PMEI, pectin methylesterase inhibitor; qua2-1, quasimodo2-1; SE, standard error; WT, wild type.