Analysis of the levels of HGA regions and saccharification efficiency in the Arabidopsis nested core collection. (A) PAM1 epitope content. Bars represent average of PAM1 epitope level ± SD (n = 3) express with respect to 1 μg PGA signal. (B) Saccharification efficiency of leaves. Bars represent average of saccharification efficiency ± SD (n ≥10). Different letters indicate statistically significant differences, according to ANOVA followed by Tukey’s test (P <0.05). The experiments were repeated three times with similar results. HGA, de-methyl-esterified homogalacturonan; PGA, polygalacturonic acid.