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Figure 1 | Biotechnology for Biofuels

Figure 1

From: Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases

Figure 1

Schematic representation of the final vector constructs used in this study. The cDNA of (a) amyA and (b) glaA were expressed in plasmids yBBHI-AmyA and yBBH1-GlaA, respectively, and (c) co-expressed in plasmid yBBHI-AmyA-GlaA under regulation of the enolase I (ENO1) promoter and terminator sequences. ori, bacterial origin of replication; bla, ampicillin-resistance gene; URA3, yeast auxotrophic marker; 2 μ, yeast 2-micron origin of replication; Bam HI, Bgl II, Eco RI and Xho I, restriction enzyme sites used for cloning.

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