Figure 1
From: Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases
Schematic representation of the final vector constructs used in this study. The cDNA of (a) amyA and (b) glaA were expressed in plasmids yBBHI-AmyA and yBBH1-GlaA, respectively, and (c) co-expressed in plasmid yBBHI-AmyA-GlaA under regulation of the enolase I (ENO1) promoter and terminator sequences. ori, bacterial origin of replication; bla, ampicillin-resistance gene; URA3, yeast auxotrophic marker; 2 μ, yeast 2-micron origin of replication; Bam HI, Bgl II, Eco RI and Xho I, restriction enzyme sites used for cloning.