Table 2 Conversion of raw starch to ethanol and byproducts by recombinant S . cerevisiae strains
From: Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases
Substrate/product | S. cerevisiae Y294 |  | S. cerevisiae Mnuα1 |  | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
(g l-1) | [AmyA] | [GlaA] | [AmyA-GlaA] |  | [AmyA] | [GlaA] | [AmyA-GlaA] |  | ||
| Â | Day 10 | Day 10 | Day 10 | Day 5 | Day 10 | Day 10 | Day 10 | Day 10 | Day 5 | Day 10 |
Substrate | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
Raw starch | 20.0 | 20.0 | 20.0 | 200.0 | 200.0 | 20.0 | 20.0 | 20.0 | 200.0 | 200.0 |
Glucose | Â | Â | Â | 5.0 | 5.0 | Â | Â | Â | 5.0 | 5.0 |
Glucose equivalent | 22.2 | 22.2 | 22.2 | 227.0 | 227.0 | 22.2 | 22.2 | 22.2 | 227.0 | 227.0 |
Products | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
Glucose | ND | ND | 0.31 ± 0.06 | ND | 23.71 ± 6.13 | ND | ND | ND | 2.21 ± 0.40 | 36.11 ± 3.44 |
Maltose | ND | ND | ND | 0.39 ± 0.04 | ND | ND | ND | ND | 0.37 ± 0.04 | ND |
Glycerol | 0.12 ± 0.01 | 0.89 ± 0.18 | 1.50 ± 0.17 | 3.17 ± 0.32 | 3.1 ± 0.13 | ND | 0.19 ± 0.03 | 0.42 ± 0.01 | 3.93 ± 0.09 | 2.63 ± 0.09 |
Acetic acid | 0.32 ± 0.01 | 0.59 ± 0.07 | 0.69 ± 0.07 | 0.65 ± 0.04 | 0.14 ± 0.04 | 0.32 ± 0.01 | 0.36 ± 0.02 | 0.3 ± 0.01 | 0.40 ± 0.03 | ND |
Ethanol | 1.27 ± 0.03 | 4.82 ± 0.58 | 6.67 ± 0.65 | 43.33 ± 5.20 | 53.02 ± 2.80 | 0.94 ± 0.15 | 5.10 ± 1.03 | 9.03 ± 0.34 | 70.07 ± 2.58 | 65.83 ± 1.67 |
CO2 | 1.22 | 4.61 | 6.38 | 41.45 | 50.71 | 0.90 | 4.88 | 8.64 | 67.02 | 62.97 |
Total carbon | 2.94 | 10.92 | 15.55 | 88.98 | 130.68 | 2.16 | 10.52 | 18.45 | 144.00 | 167.54 |
Carbon conversion rate | 13% | 49% | 70% | 39% | 58% | 10% | 47% | 83% | 63% | 74% |
Ethanol (% theoretical) | 11% | 43% | 59% | 37% | 46% | 8% | 45% | 80% | 61% | 57% |
Ethanol productivity | 0.01 | 0.02 | 0.03 | 0.36 | 0.22 | 0.00 | 0.02 | 0.04 | 0.58 | 0.27 |