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Table 2 Conversion of raw starch to ethanol and byproducts by recombinant S . cerevisiae strains

From: Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases

Substrate/product S. cerevisiae Y294   S. cerevisiae Mnuα1  
(g l-1) [AmyA] [GlaA] [AmyA-GlaA]   [AmyA] [GlaA] [AmyA-GlaA]  
  Day 10 Day 10 Day 10 Day 5 Day 10 Day 10 Day 10 Day 10 Day 5 Day 10
Substrate           
Raw starch 20.0 20.0 20.0 200.0 200.0 20.0 20.0 20.0 200.0 200.0
Glucose     5.0 5.0     5.0 5.0
Glucose equivalent 22.2 22.2 22.2 227.0 227.0 22.2 22.2 22.2 227.0 227.0
Products           
Glucose ND ND 0.31 ± 0.06 ND 23.71 ± 6.13 ND ND ND 2.21 ± 0.40 36.11 ± 3.44
Maltose ND ND ND 0.39 ± 0.04 ND ND ND ND 0.37 ± 0.04 ND
Glycerol 0.12 ± 0.01 0.89 ± 0.18 1.50 ± 0.17 3.17 ± 0.32 3.1 ± 0.13 ND 0.19 ± 0.03 0.42 ± 0.01 3.93 ± 0.09 2.63 ± 0.09
Acetic acid 0.32 ± 0.01 0.59 ± 0.07 0.69 ± 0.07 0.65 ± 0.04 0.14 ± 0.04 0.32 ± 0.01 0.36 ± 0.02 0.3 ± 0.01 0.40 ± 0.03 ND
Ethanol 1.27 ± 0.03 4.82 ± 0.58 6.67 ± 0.65 43.33 ± 5.20 53.02 ± 2.80 0.94 ± 0.15 5.10 ± 1.03 9.03 ± 0.34 70.07 ± 2.58 65.83 ± 1.67
CO2 1.22 4.61 6.38 41.45 50.71 0.90 4.88 8.64 67.02 62.97
Total carbon 2.94 10.92 15.55 88.98 130.68 2.16 10.52 18.45 144.00 167.54
Carbon conversion rate 13% 49% 70% 39% 58% 10% 47% 83% 63% 74%
Ethanol (% theoretical) 11% 43% 59% 37% 46% 8% 45% 80% 61% 57%
Ethanol productivity 0.01 0.02 0.03 0.36 0.22 0.00 0.02 0.04 0.58 0.27
  1. ND, not detected.