Effects of pH and temperature on the activity and stability of the recombinant Xyn10A. a. Optimal pH and pH stability of the Xyn10A. b. Effect of temperature on Xyn10A activity. c. The thermostability of the Xyn10A with 5 mM Ca2+. d. The thermostability of Xyn10A with different concentration of Ca2+. The residual activity was monitored, and the maximum activity was defined as 100% (a, b) or initial activity was defined as 100% (c, d). Values shown were the mean of the average of three experiments, and the variation about the mean was below 5%.