Carbohydrate-binding modules (CBMs) revisited: reduced amount of water counterbalances the need for CBMs

Table 2 Free cellulases in the hydrolysates after 48 h hydrolysis

Substrate	Enzyme mix	Consistency	Enzyme recovery^a					Cel7A recovery^a
		% (w/V)	% of free protein of total applied					% activity in solution of total applied
			Cel7A	Cel7B	Cel6A	Cel5A	Cel3A	Based on MUL activity
WS	Intact	1	29	61	13	19	110	21
		10	6	35	5	6	99	11
		20	bdl ^b	8	6	bdl ^b	4	3
	Core	1	99	100	64	90	131	85
		10	96	90	28	54	122	86
		20	60	16	0	18	23	59
A	Intact	1	27	38	23	23 ^c	101	18
		10	38	60	30	26 ^c	129	31
		20	8	15	11	bdl ^b,c	97	6
	Core	1	14 ^d	5 ^d	6 ^c,d	14 ^d	68 ^d	26 ^d
		10	92	70	80 ^c	100	127	65
		20	96	18	bdl ^b,c	82	115	74

^a enzymes free in the supernatant after 48 h hydrolysis.
^b bdl = below detection limit.
^c enzyme and Man5A together (overlapping bands).
^d enzyme recovery was low due to enzyme inactivation.
Free cellulases in the hydrolysates after 48 h hydrolysis, separated and quantified by protein measurement by SDS-PAGE. The activity of Cel7A was also quantified in the supernatants using MUL as substrate.

ISSN: 2731-3654