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Table 2 Free cellulases in the hydrolysates after 48 h hydrolysis

From: Carbohydrate-binding modules (CBMs) revisited: reduced amount of water counterbalances the need for CBMs

Substrate Enzyme mix Consistency Enzyme recoverya Cel7A recoverya
   % (w/V) % of free protein of total applied % activity in solution of total applied
    Cel7A Cel7B Cel6A Cel5A Cel3A Based on MUL activity
WS Intact 1 29 61 13 19 110 21
   10 6 35 5 6 99 11
   20 bdl b 8 6 bdl b 4 3
  Core 1 99 100 64 90 131 85
   10 96 90 28 54 122 86
   20 60 16 0 18 23 59
A Intact 1 27 38 23 23 c 101 18
   10 38 60 30 26 c 129 31
   20 8 15 11 bdl b,c 97 6
  Core 1 14 d 5 d 6 c,d 14 d 68 d 26 d
   10 92 70 80 c 100 127 65
   20 96 18 bdl b,c 82 115 74
  1. a enzymes free in the supernatant after 48 h hydrolysis.
  2. b bdl = below detection limit.
  3. c enzyme and Man5A together (overlapping bands).
  4. d enzyme recovery was low due to enzyme inactivation.
  5. Free cellulases in the hydrolysates after 48 h hydrolysis, separated and quantified by protein measurement by SDS-PAGE. The activity of Cel7A was also quantified in the supernatants using MUL as substrate.