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Table 1 Fermentation products of cellulolytic and non-cellulolytic strains isolated from ethanologenic enrichment cultures

From: Single-step ethanol production from lignocellulose using novel extremely thermophilic bacteria

Enrichment cultures used for isolation

Substrate

Isolated cellulolytic Caldicellulosiruptor sp. strains

Isolated non-cellulolytic Thermoanaerobacter sp. strains

  

Strain

Ethanol mM

Acetate mM

Lactate mM

Ethanol:Acetate:Lactate mM:mM:mM

Strain

Ethanol mM

Acetate mM

Lactate mM

Ethanol:Acetate:Lactate mM:mM:mM

#4

Cellulose (26 mM gluc.equiv.)

DIB004C

1.6 ± 0.7

4.8 ± 0.5

9.6 ± 0.1

1 : 3.0 : 6.1

DIB004G

no growth on cellulose

Cellobiose (25 mM gluc. equiv.)

0.3 ± 0.2

8.6 ± 0.1

18.8 ± 0.1

1 : 29.7 : 64.7

21.6 ± 0.6

2.4 ± 0.1

17.1 ± 0.1

1 : 0.1 : 0.8

Glucose (25 mM)

1.2 ± 0.1

6.4 ± 0.2

19.6 ± 0.5

1 : 5.2 : 15.9

23.2 ± 0.2

0.7 ± 0.1

17.9 ± 0.7

1 : 0.0 : 0.8

Xylan (31 mM xylose equiv.)

1.5 ± 0.2

9.6 ± 1.0

13.2 ± 3.1

1 : 6.5 : 9.0

no growth on xylan

Xylose (30 mM)

3.2 ± 0.4

2.5 ± 0.9

18.6 ± 0.1

1 : 0.8 : 5.9

17.1 ± 0.4

0.0 ± 0.0

10.3 ± 0.0

1 : 0.00 : 0.6

#97

Cellulose (26 mM gluc.equiv.)

no cellulolytic strains isolated

DIB097X

no growth on cellulose

Cellobiose (25 mM gluc. equiv.)

29.2 ± 0.2

5.3 ± 1.0

8.9 ± 0.3

1 : 0.2 : 0.3

Glucose (25 mM)

31.7 ± 0.7

2.8 ± 0.4

11.9 ± 0.0

1 : 0.1 : 0.4

Xylan (31 mM xylose equiv.)

19.8 ± 0.6

9.0 ± 0.1

5.1 ± 0.0

1 : 0.5 : 0.3

Xylose (30 mM)

25.5 ± 2.0

1.1 ± 0.3

8.2 ± 1.5

1 : 0.0 : 0.3

#101

Cellulose (26 mM gluc.equiv.)

DIB101C

1.1 ± 0.1

6.9 ± 0.1

6.6 ± 0.6

1 : 6.4 : 6.2

see Additional file 1: Table S2 for further details

Cellobiose (25 mM gluc. equiv.)

2.6 ± 0.5

11.1 ± 0.1

14.8 ± 0.1

1 : 4.2 : 5.6

Glucose (25 mM)

1.5 ± 0.0

9.7 ± 0.6

12.7 ± 0.5

1 : 6.6 : 8.6

Xylan (31 mM xylose equiv.)

2.2 ± 0.2

10.0 ± 0.3

9.4 ± 0.2

1 : 4.5 : 4.3

Xylose (30 mM)

5.1 ± 0.8

4.8 ± 0.7

10.5 ± 0.0

1 : 0.9 : 2.1

Control strains

Cellulose (26 mM gluc.equiv.)

Caldicellulosiruptor saccharolyticus DSM 8903

1.4 ± 0.3

5.7 ± 0.1

6.6 ± 0.1

1 : 4.1 : 4.7

Thermoanaerobacter mathranii DSM11426

no growth on cellulose

Cellobiose (25 mM gluc. equiv.)

4.7 ± 0.3

7.1 ± 1.5

14.4 ± 2.0

1 : 1.5 : 3.0

12.4 ± 0.3

7.2 ± 0.5

18.7 ± 1.2

1 : 0.6 : 1.5

Glucose (25 mM)

1.7 ± 0.2

7.8 ± 1.1

14.2 ± 3.7

1 : 4.7 : 8.6

17.2 ± 0.4

8.5 ± 0.5

17.0 ± 1.3

1 : 0.5 : 1.0

Xylan (31 mM xylose equiv.)

1.8 ± 0.3

10.2 ± 0.4

7.7 ± 0.3

1 : 5.5 : 4.2

19.9 ± 0.7

4.9 ± 0.4

2.7 ± 0.0

1 : 0.2 : 0.1

 

Xylose (30 mM)

 

2.9 ± 0.5

4.1 ± 0.8

14.7 ± 1.3

1 : 1.4 : 5.1

 

13.7 ± 0.0

4.9 ± 0.1

12.6 ± 0.6

1 : 0.4 : 0.9

  1. Cultures were grown at 72°C for 6 days in Hungate tubes without shaking. Experiments were performed in duplicates.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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