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Table 1 Fermentation products of cellulolytic and non-cellulolytic strains isolated from ethanologenic enrichment cultures

From: Single-step ethanol production from lignocellulose using novel extremely thermophilic bacteria

Enrichment cultures used for isolation Substrate Isolated cellulolytic Caldicellulosiruptor sp. strains Isolated non-cellulolytic Thermoanaerobacter sp. strains
   Strain Ethanol mM Acetate mM Lactate mM Ethanol:Acetate:Lactate mM:mM:mM Strain Ethanol mM Acetate mM Lactate mM Ethanol:Acetate:Lactate mM:mM:mM
#4 Cellulose (26 mM gluc.equiv.) DIB004C 1.6 ± 0.7 4.8 ± 0.5 9.6 ± 0.1 1 : 3.0 : 6.1 DIB004G no growth on cellulose
Cellobiose (25 mM gluc. equiv.) 0.3 ± 0.2 8.6 ± 0.1 18.8 ± 0.1 1 : 29.7 : 64.7 21.6 ± 0.6 2.4 ± 0.1 17.1 ± 0.1 1 : 0.1 : 0.8
Glucose (25 mM) 1.2 ± 0.1 6.4 ± 0.2 19.6 ± 0.5 1 : 5.2 : 15.9 23.2 ± 0.2 0.7 ± 0.1 17.9 ± 0.7 1 : 0.0 : 0.8
Xylan (31 mM xylose equiv.) 1.5 ± 0.2 9.6 ± 1.0 13.2 ± 3.1 1 : 6.5 : 9.0 no growth on xylan
Xylose (30 mM) 3.2 ± 0.4 2.5 ± 0.9 18.6 ± 0.1 1 : 0.8 : 5.9 17.1 ± 0.4 0.0 ± 0.0 10.3 ± 0.0 1 : 0.00 : 0.6
#97 Cellulose (26 mM gluc.equiv.) no cellulolytic strains isolated DIB097X no growth on cellulose
Cellobiose (25 mM gluc. equiv.) 29.2 ± 0.2 5.3 ± 1.0 8.9 ± 0.3 1 : 0.2 : 0.3
Glucose (25 mM) 31.7 ± 0.7 2.8 ± 0.4 11.9 ± 0.0 1 : 0.1 : 0.4
Xylan (31 mM xylose equiv.) 19.8 ± 0.6 9.0 ± 0.1 5.1 ± 0.0 1 : 0.5 : 0.3
Xylose (30 mM) 25.5 ± 2.0 1.1 ± 0.3 8.2 ± 1.5 1 : 0.0 : 0.3
#101 Cellulose (26 mM gluc.equiv.) DIB101C 1.1 ± 0.1 6.9 ± 0.1 6.6 ± 0.6 1 : 6.4 : 6.2 see Additional file 1: Table S2 for further details
Cellobiose (25 mM gluc. equiv.) 2.6 ± 0.5 11.1 ± 0.1 14.8 ± 0.1 1 : 4.2 : 5.6
Glucose (25 mM) 1.5 ± 0.0 9.7 ± 0.6 12.7 ± 0.5 1 : 6.6 : 8.6
Xylan (31 mM xylose equiv.) 2.2 ± 0.2 10.0 ± 0.3 9.4 ± 0.2 1 : 4.5 : 4.3
Xylose (30 mM) 5.1 ± 0.8 4.8 ± 0.7 10.5 ± 0.0 1 : 0.9 : 2.1
Control strains Cellulose (26 mM gluc.equiv.) Caldicellulosiruptor saccharolyticus DSM 8903 1.4 ± 0.3 5.7 ± 0.1 6.6 ± 0.1 1 : 4.1 : 4.7 Thermoanaerobacter mathranii DSM11426 no growth on cellulose
Cellobiose (25 mM gluc. equiv.) 4.7 ± 0.3 7.1 ± 1.5 14.4 ± 2.0 1 : 1.5 : 3.0 12.4 ± 0.3 7.2 ± 0.5 18.7 ± 1.2 1 : 0.6 : 1.5
Glucose (25 mM) 1.7 ± 0.2 7.8 ± 1.1 14.2 ± 3.7 1 : 4.7 : 8.6 17.2 ± 0.4 8.5 ± 0.5 17.0 ± 1.3 1 : 0.5 : 1.0
Xylan (31 mM xylose equiv.) 1.8 ± 0.3 10.2 ± 0.4 7.7 ± 0.3 1 : 5.5 : 4.2 19.9 ± 0.7 4.9 ± 0.4 2.7 ± 0.0 1 : 0.2 : 0.1
  Xylose (30 mM)   2.9 ± 0.5 4.1 ± 0.8 14.7 ± 1.3 1 : 1.4 : 5.1   13.7 ± 0.0 4.9 ± 0.1 12.6 ± 0.6 1 : 0.4 : 0.9
  1. Cultures were grown at 72°C for 6 days in Hungate tubes without shaking. Experiments were performed in duplicates.