Towards a metagenomic understanding on enhanced biomethane production from waste activated sludge after pH 10 pretreatment

Table 1 Statistics of 454 GS Junior pyrosequence datasets presented in this study

Sample	P30	UP30	UP40
Pretreatment	pH10 for 8d	Un-pretreated	Un-pretreated
Semi-continuous culture	30 d	30 d	40 d
Sequences count	151,676	114,694	134,522
Mean sequence length	392±120 bp	289±99 bp	425±115 bp
Archaea	8.32%	12.13%	15.13%
Bacteria	85.46%	81.82%	79.10%
Eukaryota	0.45%	0.30%	0.48%
Viruses	0.01%	0.02%	0.02%
other sequences	0.01%	0.01%	0.00%
unassigned	5.75%	5.72%	5.28%

Legends: By taxonomic classification based on M5NR database, Bacteria (82.13% on average) and Archaea (11.86% on average) are the dominant domains, with variable proportions in the bioreactor samples. UP 30 and UP40 are collected from same reactor at different time since semi-continuous culture.

ISSN: 2731-3654