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Table 3 Polysaccharide content and composition

From: Lignin biosynthesis perturbations affect secondary cell wall composition and saccharification yield in Arabidopsis thaliana

Line Cellulose Matrix polysaccharides Rhamnose Fucose Arabinose Xylose Mannose Glucose Galactose Frequency of [Me]GlcA branching of xylan % methylated GlcA on xylan
WT Col-0 38.9 ± 2.3 35.6 ± 1.4 3.4 ± 0.1 2.2 ± 0.4 12.7 ± 0.4 58.7 ± 1.3 2.8 ± 0.2 5.7 ± 1.7 14.5 ± 0.3 13.3 ± 0.6 64.9 ± 0.6
pal1-2 41.6 ± 5.4 35.7 ± 1.8 3.0 ± 0.3** 2.0 ± 0.5 13.2 ± 0.4 55.2 ± 3.9* 2.3 ± 0.3* 9.8 ± 4.8 14.5 ± 0.4 13.2 ± 0.9 66.3 ± 0.8
pal1-3 42.9 ± 4.0 37.3 ± 1.1 3.3 ± 0.2 2.3 ± 0.3 12.7 ± 0.7 56.0 ± 3.0 2.6 ± 0.2 8.9 ± 4.2 14.2 ± 0.5 13.2 ± 1.0 66.8 ± 1.0
pal2-2 41.5 ± 4.6 37.1 ± 1.5 3.5 ± 0.1 2.3 ± 0.3 12.6 ± 0.8 57.3 ± 2.7 2.9 ± 0.2 7.1 ± 4.3 14.4 ± 0.8 13.2 ± 0.6 64.2 ± 1.0
pal2-3 41.4 ± 2.5 36.8 ± 1.7 3.4 ± 0.2 2.3 ± 0.2 12.3 ± 0.8 56.7 ± 3.4 2.7 ± 0.3 8.7 ± 5.1 13.9 ± 0.7 13.1 ± 0.9 64.6 ± 1.1
c4h-2 36.3 ± 2.1 42.9 ± 1.7** 3.6 ± 0.1 2.7 ± 0.4* 15.4 ± 0.9** 57.0 ± 1.9 2.8 ± 0.1 3.1 ± 0.2 15.4 ± 0.9* 12.6 ± 0.5 80.3 ± 1.2**
c4h-3 40.7 ± 3.0 38.7 ± 0.9** 3.3 ± 0.3 2.5 ± 0.4 14.4 ± 0.9** 59.0 ± 1.2 2.8 ± 0.4 2.7 ± 0.6 15.5 ± 1.0* 12.9 ± 0.6 72.1 ± 1.5**
4cl1-1 41.4 ± 2.6 37.8 ± 1.4** 3.5 ± 0.2 2.3 ± 0.5 13.3 ± 0.4 58.9 ± 1.5 2.9 ± 0.2 4.3 ± 1.3 14.8 ± 0.3 13.0 ± 0.4 74.7 ± 1.8**
4cl1-2 40.7 ± 2.8 39.4 ± 1.2** 3.6 ± 0.1 2.2 ± 0.4 12.4 ± 0.7 58.6 ± 1.6 3.0 ± 0.1 6.2 ± 1.8 14.1 ± 0.6 13.1 ± 1.0 74.1 ± 1.5**
4cl2-1 37.8 ± 2.2 38.9 ± 1.4** 3.7 ± 0.3** 2.3 ± 0.2 12.1 ± 1.0 56.7 ± 3.3 2.8 ± 1.1 8.5 ± 6.5 13.8 ± 0.9 12.8 ± 0.4 64.5 ± 0.6
4cl2-3 38.5 ± 2.3 38.3 ± 1.3** 3.7 ± 0.2** 2.1 ± 0.4 12.0 ± 0.4 57.4 ± 2.5 3.1 ± 0.2 7.6 ± 2.9 14.0 ± 0.3 12.7 ± 0.6 63.8 ± 1.2
ccoaomt1-3 39.8 ± 1.7 39.6 ± 1.4** 3.8 ± 0.2** 2.4 ± 0.1 12.4 ± 0.6 59.6 ± 1.2 3.2 ± 0.2 3.8 ± 0.8 14.9 ± 0.4 13.4 ± 0.7 68.8 ± 2.1**
ccoaomt1-5 41.0 ± 2.6 38.4 ± 1.4** 3.4 ± 0.1 2.2 ± 0.4 13.5 ± 1.4 57.1 ± 5.1 3.0 ± 0.6 5.0 ± 2.9 15.8 ± 1.4** 13.5 ± 0.4 73.5 ± 0.6**
ccr1-3 23.4 ± 3.5** 46.6 ± 1.5** 3.1 ± 0.3 1.8 ± 0.4 19.2 ± 1.3** 50.3 ± 2.2** 2.4 ± 0.3 4.9 ± 3.4 18.2 ± 1.0** 14.2 ± 0.4 55.2 ± 1.1**
ccr1-6 30.9 ± 2.6** 44.6 ± 1.3** 3.6 ± 0.3 2.2 ± 0.5 15.7 ± 0.4** 54.1 ± 1.9** 2.7 ± 0.1 5.6 ± 2.4 16.0 ± 0.5* 14.5 ± 0.4 54.9 ± 1.8**
f5h1-2 31.7 ± 2.0** 35.7 ± 1.3 3.6 ± 0.1 2.1 ± 0.5 12.6 ± 0.4 57.0 ± 1.9 3.0 ± 0.1 7.4 ± 2.2 14.4 ± 0.2 13.0 ± 0.7 66.9 ± 0.6
f5h1-4 32.8 ± 3.2** 35.8 ± 1.3 3.5 ± 0.2 2.0 ± 0.4 12.5 ± 0.9 55.6 ± 3.7 2.8 ± 0.3 9.2 ± 5.5 14.4 ± 0.7 13.0 ± 0.3 66.3 ± 0.7
comt-1 33.4 ± 1.8** 37.2 ± 1.7 3.4 ± 0.2 2.1 ± 0.4 12.7 ± 0.7 56.5 ± 2.6 2.9 ± 0.2 7.6 ± 3.6 14.8 ± 0.4 12.7 ± 0.4 65.6 ± 1.9
comt-4 33.2 ± 2.1** 36.1 ± 0.9 3.2 ± 0.1 2.2 ± 0.4 12.8 ± 0.5 55.2 ± 2.6* 2.8 ± 0.2 7.9 ± 3.4 15.9 ± 0.5** 13.1 ± 0.8 65.2 ± 0.6
cad6-1 40.6 ± 3.2 34.8 ± 1.2 3.2 ± 0.1 2.3 ± 0.5 13.5 ± 0.9 58.8 ± 1.6 2.6 ± 0.1 5.1 ± 2.7 14.5 ± 0.5 13.0 ± 0.5 66.2 ± 0.7
cad6-4 38.9 ± 3.3 36.2 ± 1.1 3.3 ± 0.1 2.2 ± 0.3 12.7 ± 0.5 57.5 ± 1.8 2.8 ± 0.1 7.1 ± 2.4 14.4 ± 0.6 13.0 ± 0.7 66.4 ± 0.5
  1. Cellulose and matrix polysaccharide contents for the 20 lignin mutants (n = 8) and wild-type (n = 16) (± SD) were expressed as percentage CWR. Matrix polysaccharide content was determined gravimetrically based on a TFA extraction and its composition was expressed as mol%. Xylan branching frequency and the percentage methylation of GlcA were determined by DASH. GlcA: glucuronic acid. Increases and decreases compared to the wild-type are indicated in bold and italics, respectively. * 0.001 < p < 0.01; ** p < 0.001 (Dunnett-Hsu adjusted t-test).