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Table 3 Polysaccharide content and composition

From: Lignin biosynthesis perturbations affect secondary cell wall composition and saccharification yield in Arabidopsis thaliana

Line

Cellulose

Matrix polysaccharides

Rhamnose

Fucose

Arabinose

Xylose

Mannose

Glucose

Galactose

Frequency of [Me]GlcA branching of xylan

% methylated GlcA on xylan

WT Col-0

38.9 ± 2.3

35.6 ± 1.4

3.4 ± 0.1

2.2 ± 0.4

12.7 ± 0.4

58.7 ± 1.3

2.8 ± 0.2

5.7 ± 1.7

14.5 ± 0.3

13.3 ± 0.6

64.9 ± 0.6

pal1-2

41.6 ± 5.4

35.7 ± 1.8

3.0 ± 0.3**

2.0 ± 0.5

13.2 ± 0.4

55.2 ± 3.9*

2.3 ± 0.3*

9.8 ± 4.8

14.5 ± 0.4

13.2 ± 0.9

66.3 ± 0.8

pal1-3

42.9 ± 4.0

37.3 ± 1.1

3.3 ± 0.2

2.3 ± 0.3

12.7 ± 0.7

56.0 ± 3.0

2.6 ± 0.2

8.9 ± 4.2

14.2 ± 0.5

13.2 ± 1.0

66.8 ± 1.0

pal2-2

41.5 ± 4.6

37.1 ± 1.5

3.5 ± 0.1

2.3 ± 0.3

12.6 ± 0.8

57.3 ± 2.7

2.9 ± 0.2

7.1 ± 4.3

14.4 ± 0.8

13.2 ± 0.6

64.2 ± 1.0

pal2-3

41.4 ± 2.5

36.8 ± 1.7

3.4 ± 0.2

2.3 ± 0.2

12.3 ± 0.8

56.7 ± 3.4

2.7 ± 0.3

8.7 ± 5.1

13.9 ± 0.7

13.1 ± 0.9

64.6 ± 1.1

c4h-2

36.3 ± 2.1

42.9 ± 1.7**

3.6 ± 0.1

2.7 ± 0.4*

15.4 ± 0.9**

57.0 ± 1.9

2.8 ± 0.1

3.1 ± 0.2

15.4 ± 0.9*

12.6 ± 0.5

80.3 ± 1.2**

c4h-3

40.7 ± 3.0

38.7 ± 0.9**

3.3 ± 0.3

2.5 ± 0.4

14.4 ± 0.9**

59.0 ± 1.2

2.8 ± 0.4

2.7 ± 0.6

15.5 ± 1.0*

12.9 ± 0.6

72.1 ± 1.5**

4cl1-1

41.4 ± 2.6

37.8 ± 1.4**

3.5 ± 0.2

2.3 ± 0.5

13.3 ± 0.4

58.9 ± 1.5

2.9 ± 0.2

4.3 ± 1.3

14.8 ± 0.3

13.0 ± 0.4

74.7 ± 1.8**

4cl1-2

40.7 ± 2.8

39.4 ± 1.2**

3.6 ± 0.1

2.2 ± 0.4

12.4 ± 0.7

58.6 ± 1.6

3.0 ± 0.1

6.2 ± 1.8

14.1 ± 0.6

13.1 ± 1.0

74.1 ± 1.5**

4cl2-1

37.8 ± 2.2

38.9 ± 1.4**

3.7 ± 0.3**

2.3 ± 0.2

12.1 ± 1.0

56.7 ± 3.3

2.8 ± 1.1

8.5 ± 6.5

13.8 ± 0.9

12.8 ± 0.4

64.5 ± 0.6

4cl2-3

38.5 ± 2.3

38.3 ± 1.3**

3.7 ± 0.2**

2.1 ± 0.4

12.0 ± 0.4

57.4 ± 2.5

3.1 ± 0.2

7.6 ± 2.9

14.0 ± 0.3

12.7 ± 0.6

63.8 ± 1.2

ccoaomt1-3

39.8 ± 1.7

39.6 ± 1.4**

3.8 ± 0.2**

2.4 ± 0.1

12.4 ± 0.6

59.6 ± 1.2

3.2 ± 0.2

3.8 ± 0.8

14.9 ± 0.4

13.4 ± 0.7

68.8 ± 2.1**

ccoaomt1-5

41.0 ± 2.6

38.4 ± 1.4**

3.4 ± 0.1

2.2 ± 0.4

13.5 ± 1.4

57.1 ± 5.1

3.0 ± 0.6

5.0 ± 2.9

15.8 ± 1.4**

13.5 ± 0.4

73.5 ± 0.6**

ccr1-3

23.4 ± 3.5**

46.6 ± 1.5**

3.1 ± 0.3

1.8 ± 0.4

19.2 ± 1.3**

50.3 ± 2.2**

2.4 ± 0.3

4.9 ± 3.4

18.2 ± 1.0**

14.2 ± 0.4

55.2 ± 1.1**

ccr1-6

30.9 ± 2.6**

44.6 ± 1.3**

3.6 ± 0.3

2.2 ± 0.5

15.7 ± 0.4**

54.1 ± 1.9**

2.7 ± 0.1

5.6 ± 2.4

16.0 ± 0.5*

14.5 ± 0.4

54.9 ± 1.8**

f5h1-2

31.7 ± 2.0**

35.7 ± 1.3

3.6 ± 0.1

2.1 ± 0.5

12.6 ± 0.4

57.0 ± 1.9

3.0 ± 0.1

7.4 ± 2.2

14.4 ± 0.2

13.0 ± 0.7

66.9 ± 0.6

f5h1-4

32.8 ± 3.2**

35.8 ± 1.3

3.5 ± 0.2

2.0 ± 0.4

12.5 ± 0.9

55.6 ± 3.7

2.8 ± 0.3

9.2 ± 5.5

14.4 ± 0.7

13.0 ± 0.3

66.3 ± 0.7

comt-1

33.4 ± 1.8**

37.2 ± 1.7

3.4 ± 0.2

2.1 ± 0.4

12.7 ± 0.7

56.5 ± 2.6

2.9 ± 0.2

7.6 ± 3.6

14.8 ± 0.4

12.7 ± 0.4

65.6 ± 1.9

comt-4

33.2 ± 2.1**

36.1 ± 0.9

3.2 ± 0.1

2.2 ± 0.4

12.8 ± 0.5

55.2 ± 2.6*

2.8 ± 0.2

7.9 ± 3.4

15.9 ± 0.5**

13.1 ± 0.8

65.2 ± 0.6

cad6-1

40.6 ± 3.2

34.8 ± 1.2

3.2 ± 0.1

2.3 ± 0.5

13.5 ± 0.9

58.8 ± 1.6

2.6 ± 0.1

5.1 ± 2.7

14.5 ± 0.5

13.0 ± 0.5

66.2 ± 0.7

cad6-4

38.9 ± 3.3

36.2 ± 1.1

3.3 ± 0.1

2.2 ± 0.3

12.7 ± 0.5

57.5 ± 1.8

2.8 ± 0.1

7.1 ± 2.4

14.4 ± 0.6

13.0 ± 0.7

66.4 ± 0.5

  1. Cellulose and matrix polysaccharide contents for the 20 lignin mutants (n = 8) and wild-type (n = 16) (± SD) were expressed as percentage CWR. Matrix polysaccharide content was determined gravimetrically based on a TFA extraction and its composition was expressed as mol%. Xylan branching frequency and the percentage methylation of GlcA were determined by DASH. GlcA: glucuronic acid. Increases and decreases compared to the wild-type are indicated in bold and italics, respectively. * 0.001 < p < 0.01; ** p < 0.001 (Dunnett-Hsu adjusted t-test).