Table 1 Set of 35 genes whose deletion results in n -butanol sensitivity in both BY4741 and CEN.PK113-7D genetic backgrounds

PRE9

Alpha 3 subunit of the 20S proteasome. PRE9 encodes the only nonessential proteasome subunit.

UMP1

Chaperone required for correct maturation of the 20S proteasome.

YLR224W

Subunit of the SCF ubiquitin ligase complexes, responsible of recognizing misfolded proteins.

UBP3

Ubiquitin-specific protease and its cofactor, respectively. Responsible of the deubiquitination of proteins. Important to maintain the pull of free ubiquitin in the cells.

BRE5

STP22

Components of the ESCRT-I, -II and –II complexes, respectively. Responsible of sorting ubiquitinated membrane proteins into Multivesicular Bodies (MVB) for their degradation in the vacuole.

SNF8

DID4

BRO1

Responsible of deubiquitination in the MBV. Important to maintain the pull of free ubiquitin.

VPS34

Vps34 and Vps15 form a complex responsible for the synthesis of phosphatidylinositol 3-phosphate, involved in endosomal membrane trafficking and in the regulation of protein sorting.

VPS15

PIH1

Component of the conserved R2TP complex. Interacts with Hsp90 to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II.

SWI6

Transcription cofactor required for the unfolded protein response.

GET1

Subunits of the GET complex. Involved in the insertion of tail anchored proteins into the ER. Tail anchored proteins play a role in vesicular traffic and folding or degradation of membrane proteins.

GET2

VMA7

Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+−ATPase, and peripheral membrane protein that is required for vacuolar H+−ATPase function, respectively.

VMA22

SHE4

Protein that regulates myosin function; involved in endocytosis.

GND1

6-phosphogluconate dehydrogenase, required for adaptation to oxidative stress.

ANP1

Subunit of the alpha-1,6 mannosyltransferase complex. Involved in osmotic sensitivity.

GEP5

Protein of unknown function, required for mitochondrial genome maintenance.

THP2

Subunits of the THO complex. Involved in transcription elongation by RNA polymerase II and in telomere maintenance.

MFT1

SLA1

Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton.

SEC28

Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic.

MSE1

Mitochondrial glutamyl-tRNA synthetase.

NKP2

Non-essential kinetochore protein, subunit of the Ctf19 central kinetochore complex.

ALD6

Cytosolic aldehyde dehydrogenase, required for conversion of acetaldehyde to acetate.

SNT309

Member of the NineTeen Complex. Involved in splicing of nuclear RNAs via the spliceosome.

REG1

Regulatory subunit of Glc7p, involved in negative regulation of glucose-repressible genes.

HTL1

Component of the RSC chromatin remodeling complex. involved in telomere maintenance.

POL32

Third subunit of DNA polymerase delta, involved in chromosomal DNA replication.

DHH1

Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping.

VRP1

Actin-associated protein involved in cytoskeletal organization and cytokinesis.

HOM2

Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis.