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Table 1 Bacterial strains and plasmids used in this study
| Plasmid or strain | Relevant genotype or description | Reference |
|---|---|---|
| Plasmids | ||
| pACYCDuet-1 | P15A origin; CmR; PT7 | Novagen |
| pTrcHis2B | ColE1 origin; AmpR; Ptrc | Invitrogen |
| pCOLADuet-1 | ColA origin; KanR; PT7 | Novagen |
| pISP212 | P15A origin; CmR; PT7::HMGS(Sc)-tHMGR(Sc) | This study |
| pISP214 | P15A origin; CmR; PT7::mvaS(Ef)-mvaE(Ef) | This study |
| pISP214m | P15A origin; CmR; PT7::mvaS A110G-mvaE(Ef) | This study |
| pTrcLower | ColE1 origin; AmpR; Ptrc:: ERG12(Sc)-ERG8(Sc)-ERG19(Sc)-IDI1(Sc) | [19] |
| pISP9 | ColE1 origin; AmpR; Ptrc:: ERG12(Sc)-ERG8(Sc)-ERG19(Sc) | This study |
| pYY11 | ColA origin; KanR; PT7:: ‘phoA | This study |
| pYY12 | ColA origin; KanR; PT7:: ‘DPP1 | This study |
| pYY13 | ColA origin; KanR; PT7:: ‘LPP1 | This study |
| pYY14 | ColA origin; KanR; PT7:: phoE | This study |
| pYY15 | ColA origin; KanR; PT7::BsNudF | This study |
| pYY16 | ColA origin; KanR; PT7::EcNudF | This study |
| pYY26 | ColA origin; KanR; PT7::NudC | This study |
| pYY36 | ColA origin; KanR; PT7::yggV | This study |
| pYY46 | ColA origin; KanR; PT7::hisl | This study |
| pYY56 | ColA origin; KanR; PT7::lpxH | This study |
| pYY66 | ColA origin; KanR; PT7::ppa | This study |
| pYY76 | ColA origin; KanR; PT7::cdh | This study |
| Strains | ||
| DH5α | deoR, recA1, endA1, hsdR17(rk-,mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 | Takara |
| BL21(DE3) | E. coli B dcm ompT hsdS(rB-mB-) gal | Invitrogen |
| W 3110 | F- λ- rph-1 INV(rrnD, rrnE) | [24] |
| DNF(DE3) | W3110: ∆EcNudF(DE3) | This study |
| DNFYV(DE3) | W3110: ∆EcNudF∆yggV(DE3) | This study |
| ISP212 | BL21(DE3) harboring pISP212 | This study |
| ISP214 | BL21(DE3) harboring pISP214 | This study |
| ISP214m | BL21(DE3) harboring pISP214m | This study |
| ISP2148 | BL21(DE3) harboring pISP214 and pTrcLower | This study |
| ISP2149 | BL21(DE3) harboring pISP214 and pISP9 | This study |
| YY118 | BL21(DE3) harboring pISP214, pTrcLower and pYY11 | This study |
| YY128 | BL21(DE3) harboring pISP214, pTrcLower and pYY12 | This study |
| YY138 | BL21(DE3) harboring pISP214, pTrcLower and pYY13 | This study |
| YY148 | BL21(DE3) harboring pISP214, pTrcLower and pYY14 | This study |
| YY158 | BL21(DE3) harboring pISP214, pTrcLower and pYY15 | This study |
| YY168 | BL21(DE3) harboring pISP214, pTrcLower and pYY16 | This study |
| YY119 | BL21(DE3) harboring pISP214, pISP9 and pYY11 | This study |
| YY129 | BL21(DE3) harboring pISP214, pISP9 and pYY12 | This study |
| YY139 | BL21(DE3) harboring pISP214, pISP9 and pYY13 | This study |
| YY149 | BL21(DE3) harboring pISP214, pISP9 and pYY14 | This study |
| YY159 | BL21(DE3) harboring pISP214, pISP9 and pYY15 | This study |
| YY169 | BL21(DE3) harboring pISP214, pISP9 and pYY16 | This study |
| YY159m | BL21(DE3) harboring pISP214m, pISP9 and pYY15 | This study |
| YY168m | BL21(DE3) harboring pISP214m, pTrcLower and pYY16 | This study |
| YY268 | BL21(DE3) harboring pISP214, pTrcLower and pYY26 | This study |
| YY368 | BL21(DE3) harboring pISP214, pTrcLower and pYY36 | This study |
| YY468 | BL21(DE3) harboring pISP214, pTrcLower and pYY46 | This study |
| YY568 | BL21(DE3) harboring pISP214, pTrcLower and pYY56 | This study |
| YY668 | BL21(DE3) harboring pISP214, pTrcLower and pYY66 | This study |
| YY768 | BL21(DE3) harboring pISP214, pTrcLower and pYY76 | This study |
