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Table 1 Bacterial strains and plasmids used in this study

From: Metabolic engineering of Escherichia coli for high-specificity production of isoprenol and prenol as next generation of biofuels

Plasmid or strain Relevant genotype or description Reference
Plasmids
pACYCDuet-1 P15A origin; CmR; PT7 Novagen
pTrcHis2B ColE1 origin; AmpR; Ptrc Invitrogen
pCOLADuet-1 ColA origin; KanR; PT7 Novagen
pISP212 P15A origin; CmR; PT7::HMGS(Sc)-tHMGR(Sc) This study
pISP214 P15A origin; CmR; PT7::mvaS(Ef)-mvaE(Ef) This study
pISP214m P15A origin; CmR; PT7::mvaS A110G-mvaE(Ef) This study
pTrcLower ColE1 origin; AmpR; Ptrc:: ERG12(Sc)-ERG8(Sc)-ERG19(Sc)-IDI1(Sc) [19]
pISP9 ColE1 origin; AmpR; Ptrc:: ERG12(Sc)-ERG8(Sc)-ERG19(Sc) This study
pYY11 ColA origin; KanR; PT7:: ‘phoA This study
pYY12 ColA origin; KanR; PT7:: ‘DPP1 This study
pYY13 ColA origin; KanR; PT7:: ‘LPP1 This study
pYY14 ColA origin; KanR; PT7:: phoE This study
pYY15 ColA origin; KanR; PT7::BsNudF This study
pYY16 ColA origin; KanR; PT7::EcNudF This study
pYY26 ColA origin; KanR; PT7::NudC This study
pYY36 ColA origin; KanR; PT7::yggV This study
pYY46 ColA origin; KanR; PT7::hisl This study
pYY56 ColA origin; KanR; PT7::lpxH This study
pYY66 ColA origin; KanR; PT7::ppa This study
pYY76 ColA origin; KanR; PT7::cdh This study
Strains
DH5α deoR, recA1, endA1, hsdR17(rk-,mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 Takara
BL21(DE3) E. coli B dcm ompT hsdS(rB-mB-) gal Invitrogen
W 3110 F- λ- rph-1 INV(rrnD, rrnE) [24]
DNF(DE3) W3110: ∆EcNudF(DE3) This study
DNFYV(DE3) W3110: ∆EcNudFyggV(DE3) This study
ISP212 BL21(DE3) harboring pISP212 This study
ISP214 BL21(DE3) harboring pISP214 This study
ISP214m BL21(DE3) harboring pISP214m This study
ISP2148 BL21(DE3) harboring pISP214 and pTrcLower This study
ISP2149 BL21(DE3) harboring pISP214 and pISP9 This study
YY118 BL21(DE3) harboring pISP214, pTrcLower and pYY11 This study
YY128 BL21(DE3) harboring pISP214, pTrcLower and pYY12 This study
YY138 BL21(DE3) harboring pISP214, pTrcLower and pYY13 This study
YY148 BL21(DE3) harboring pISP214, pTrcLower and pYY14 This study
YY158 BL21(DE3) harboring pISP214, pTrcLower and pYY15 This study
YY168 BL21(DE3) harboring pISP214, pTrcLower and pYY16 This study
YY119 BL21(DE3) harboring pISP214, pISP9 and pYY11 This study
YY129 BL21(DE3) harboring pISP214, pISP9 and pYY12 This study
YY139 BL21(DE3) harboring pISP214, pISP9 and pYY13 This study
YY149 BL21(DE3) harboring pISP214, pISP9 and pYY14 This study
YY159 BL21(DE3) harboring pISP214, pISP9 and pYY15 This study
YY169 BL21(DE3) harboring pISP214, pISP9 and pYY16 This study
YY159m BL21(DE3) harboring pISP214m, pISP9 and pYY15 This study
YY168m BL21(DE3) harboring pISP214m, pTrcLower and pYY16 This study
YY268 BL21(DE3) harboring pISP214, pTrcLower and pYY26 This study
YY368 BL21(DE3) harboring pISP214, pTrcLower and pYY36 This study
YY468 BL21(DE3) harboring pISP214, pTrcLower and pYY46 This study
YY568 BL21(DE3) harboring pISP214, pTrcLower and pYY56 This study
YY668 BL21(DE3) harboring pISP214, pTrcLower and pYY66 This study
YY768 BL21(DE3) harboring pISP214, pTrcLower and pYY76 This study