|
Plasmids
|
|
pACYCDuet-1
|
P15A origin; CmR; PT7
|
Novagen
|
|
pTrcHis2B
|
ColE1 origin; AmpR; Ptrc
|
Invitrogen
|
|
pCOLADuet-1
|
ColA origin; KanR; PT7
|
Novagen
|
|
pISP212
|
P15A origin; CmR; PT7::HMGS(Sc)-tHMGR(Sc)
|
This study
|
|
pISP214
|
P15A origin; CmR; PT7::mvaS(Ef)-mvaE(Ef)
|
This study
|
|
pISP214m
|
P15A origin; CmR; PT7::mvaS A110G-mvaE(Ef)
|
This study
|
|
pTrcLower
|
ColE1 origin; AmpR; Ptrc:: ERG12(Sc)-ERG8(Sc)-ERG19(Sc)-IDI1(Sc)
|
[19]
|
|
pISP9
|
ColE1 origin; AmpR; Ptrc:: ERG12(Sc)-ERG8(Sc)-ERG19(Sc)
|
This study
|
|
pYY11
|
ColA origin; KanR; PT7:: ‘phoA
|
This study
|
|
pYY12
|
ColA origin; KanR; PT7:: ‘DPP1
|
This study
|
|
pYY13
|
ColA origin; KanR; PT7:: ‘LPP1
|
This study
|
|
pYY14
|
ColA origin; KanR; PT7:: phoE
|
This study
|
|
pYY15
|
ColA origin; KanR; PT7::BsNudF
|
This study
|
|
pYY16
|
ColA origin; KanR; PT7::EcNudF
|
This study
|
|
pYY26
|
ColA origin; KanR; PT7::NudC
|
This study
|
|
pYY36
|
ColA origin; KanR; PT7::yggV
|
This study
|
|
pYY46
|
ColA origin; KanR; PT7::hisl
|
This study
|
|
pYY56
|
ColA origin; KanR; PT7::lpxH
|
This study
|
|
pYY66
|
ColA origin; KanR; PT7::ppa
|
This study
|
|
pYY76
|
ColA origin; KanR; PT7::cdh
|
This study
|
|
Strains
|
|
DH5α
|
deoR, recA1, endA1, hsdR17(rk-,mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1
|
Takara
|
|
BL21(DE3)
|
E. coli B dcm ompT hsdS(rB-mB-) gal
|
Invitrogen
|
|
W 3110
|
F- λ- rph-1 INV(rrnD, rrnE)
|
[24]
|
|
DNF(DE3)
|
W3110: ∆EcNudF(DE3)
|
This study
|
|
DNFYV(DE3)
|
W3110: ∆EcNudF∆yggV(DE3)
|
This study
|
|
ISP212
|
BL21(DE3) harboring pISP212
|
This study
|
|
ISP214
|
BL21(DE3) harboring pISP214
|
This study
|
|
ISP214m
|
BL21(DE3) harboring pISP214m
|
This study
|
|
ISP2148
|
BL21(DE3) harboring pISP214 and pTrcLower
|
This study
|
|
ISP2149
|
BL21(DE3) harboring pISP214 and pISP9
|
This study
|
|
YY118
|
BL21(DE3) harboring pISP214, pTrcLower and pYY11
|
This study
|
|
YY128
|
BL21(DE3) harboring pISP214, pTrcLower and pYY12
|
This study
|
|
YY138
|
BL21(DE3) harboring pISP214, pTrcLower and pYY13
|
This study
|
|
YY148
|
BL21(DE3) harboring pISP214, pTrcLower and pYY14
|
This study
|
|
YY158
|
BL21(DE3) harboring pISP214, pTrcLower and pYY15
|
This study
|
|
YY168
|
BL21(DE3) harboring pISP214, pTrcLower and pYY16
|
This study
|
|
YY119
|
BL21(DE3) harboring pISP214, pISP9 and pYY11
|
This study
|
|
YY129
|
BL21(DE3) harboring pISP214, pISP9 and pYY12
|
This study
|
|
YY139
|
BL21(DE3) harboring pISP214, pISP9 and pYY13
|
This study
|
|
YY149
|
BL21(DE3) harboring pISP214, pISP9 and pYY14
|
This study
|
|
YY159
|
BL21(DE3) harboring pISP214, pISP9 and pYY15
|
This study
|
|
YY169
|
BL21(DE3) harboring pISP214, pISP9 and pYY16
|
This study
|
|
YY159m
|
BL21(DE3) harboring pISP214m, pISP9 and pYY15
|
This study
|
|
YY168m
|
BL21(DE3) harboring pISP214m, pTrcLower and pYY16
|
This study
|
|
YY268
|
BL21(DE3) harboring pISP214, pTrcLower and pYY26
|
This study
|
|
YY368
|
BL21(DE3) harboring pISP214, pTrcLower and pYY36
|
This study
|
|
YY468
|
BL21(DE3) harboring pISP214, pTrcLower and pYY46
|
This study
|
|
YY568
|
BL21(DE3) harboring pISP214, pTrcLower and pYY56
|
This study
|
|
YY668
|
BL21(DE3) harboring pISP214, pTrcLower and pYY66
|
This study
|
|
YY768
|
BL21(DE3) harboring pISP214, pTrcLower and pYY76
|
This study
|
