Figure 5
From: Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene
Effect of different organic nitrogen source on α-pinene production by YJM28. A: beef extract (Aladdin, ○); B: beef extract (Beijing AoBoXing Bio-Tech Co., Ltd, ●); C: beef extract powder (MDBio, Inc, ■); D: beef extract powder (Beijing AoBoXing Bio-Tech Co., Ltd, ▼); E: beef extract (Beijing Shuangxuan Microbe Culture Medium Products Factory, ◄); F: beef extract (Sinopharm Chemical Reagent Co., Ltd, ►); G: beef extract (solarbio, ♦). When OD600 reached 0.6-0.9, cultures were induced at 30°C for 56 h using 0.25 mM IPTG. The experiment was performed in triplicate.