Figure 2

Analysis of transcript levels of MIHCs-encoding genes and xyr1 in T. reesei RutC30 and Iogen-M8. The xylanase overexpression strain Iogen-M8 (A-E) and its ancestor strain Rut-C30 (F-J) were precultured on glycerol and thereafter transferred to MA media without carbon source (NC) or containing 50 mM (w/v) glucose (G) or D-xylose (XO) at the indicated concentration, or 1.5 mM sophorose (SO) as an inducer. Samples were taken directly before transfer of mycelium (PRE), after 3 h of incubation (light grey bars), and after 6 h of incubation (dark grey bars). Transcription analyses of indicated genes from both strains were performed via qPCR using sar1 and act transcript levels for normalization. Results are given as relative transcript ratios in logarithmic scale (lg). The values provided in the figures are means from three biological experiments. Error bars indicate standard deviations. Transcript levels always refer to the reference sample (wild-type QM6a, NC 3 h).