Spatial accumulation of XynA in cv. 81V9 T
transplastomic plants transformed with different CECs. A. Schematic representation of the sampling procedure to obtain spatial accumulation pattern of XynA expressed from different CECs in mature T1 transplastomic plants. Samples from ten leaves (top to bottom) were extracted using equal sample weight/buffer volume (w/v) ratio and equal amounts of extracts (each lane represents 4.0 mg of extracted leaf tissue) were analysed by immunoblotting after SDS-PAGE (B). The leaf #3 corresponds to the size of a leaf used for initial expression analyses (indicated with an arrow). Untransformed (WT) plants were used as a negative control. Known amounts (in ng) of a c-myc-tagged control protein are indicated above the standard curve lanes.