Optimization of transplastomic production of hemicellulases in tobacco: effects of expression cassette configuration and tobacco cultivar used as production platform on recombinant protein yields

Table 3 Xylanolytic activity of crude plant extracts from mature green leaves (GL) and senescing leaves (SL) of cv. I64 transplastomic lines expressing XynA, Xyn10A and Xyn11B

	Enzyme Crude Extract
	XynA		Xyn10A		Xyn11B		WT control
Leaf developmental stage	GL	SL	GL	SL	GL	SL	GL	SL
Enzyme in reaction (μg)	1.7±0.15	1.5±0.5	45.9±4.3	11.3±1.1	83.2±1.9	16.8±0.3	-	-
Generated Xylose (μMole)	146.1±4.3	245.4±7.1	169.6±2.9	319.33±5.5	159.1±2.4	209.67±4.11	0.7±0.4	8.5±2.1
% Conversion*	21.8	36.6	25.3	47.7	23.7	31.3	0.1	1.3
Generated Xylose (μMole)/enzyme (μg)	85.9	163.6	3.7	28.3	1.9	12.5	-	-

Crude extracts (400 μl, representing 40 mg of extracted leaf tissue) were added to 10 ml of 1% (w/v) xylan and incubated 24 hours at 40°C with shaking. Enzyme in reaction and generated xylose values represent means of 3 different extraction repeats ± SEM.
* Initial amount of xylan in reaction is 670 μMole (0.1 g; >90% xylose residues).

ISSN: 2731-3654