Comparative transcriptome analysis to investigate the high starch accumulation of duckweed (Landoltia punctata) under nutrient starvation

Table 3 Functional enrichment of differentially expressed transcripts among three samples

Sub-ontology	GO terms	Reference	0 vs 2	pval	p.adj
Biology process	primary metabolic process	11,575	445	0.00011	0.00399
	interspecies interaction between organisms	77	10	0.00022	0.00784
cellular component	intracellular part	12,026	524	5.66E-05	0.00125
	membrane-bounded organelle	9,551	432	0.00017	0.00363
	intracellular organelle	10,868	473	0.00051	0.01122
molecular function	transferase activity	5,774	298	1.91E-09	2.87E-08
Sub-ontology	GO terms	Reference	0 vs 24	pval	p.adj
Biology process	primary metabolic process	11,575	994	1.71E-09	6.15E-08
	cellular metabolic process	8,913	753	0.00046	0.01653
cellular component	membrane-bounded organelle	9,551	883	8.48E-07	1.87E-05
	intracellular part	12,026	1,037	3.97E-05	0.00087
	intracellular organelle	10,868	947	0.00016	0.00360
	vesicle	1,648	181	0.00171	0.03755
molecular function	transferase activity	5,774	579	1.19E-12	1.79E-11
	hydrolase activity	5,415	490	0.00018	0.0027
	nucleotide binding	5,540	490	0.00092	0.01375
Sub-ontology	GO terms	Reference	2 vs 24	pval	p.adj
Biology process	primary metabolic process	11,575	866	4.61E-09	1.66E-07
	cellular metabolic process	8,913	649	0.00136	0.04901
cellular component	intracellular part	12,026	889	7.02E-05	0.00154
	intracellular organelle	10,868	805	0.00068	0.01499
molecular function	transferase activity	5,774	506	6.51E-12	9.76E-11
	hydrolase activity	5,415	458	6.68E-08	1.00E-06

GO annotation information of differentially expressed transcripts was clustered into three sub-ontologies, including molecular function, biological process, and cellular component [59]. Then hypergeometric test were performed with threshold value of padj ≤0.05 in each sub-ontology to indentify significant enriched GO terms. Reference means the transcripts annotated to the sub-ontology in the whole transcriptome.

ISSN: 2731-3654