Overcoming restriction as a barrier to DNA transformation in Caldicellulosiruptor species results in efficient marker replacement

Table 1 Susceptibility of Caldicellulosiruptor species chromosomal DNA to restriction endonuclease digestion

Source	Restriction endonucleases used for digestion
	AluI	BamHI	BspEI	EcoRI	HaeIII	HhaI	HpaII	MboI	MspI
C. bescii DSM6725	+	-	-	+	-	+	+	+	+
C. hydrothermalis DSM 18901	+	-	-	+	-	+	+	+	+
C. kristjansonii DSM12137	+	-	-	+	-	+	+	-	+
C. saccharolyticus DSM8903	+	-	-	+	-	+	+	-	+
C. obsidiansis ATCC BAA-2073	+	-	-	+	+	+	+	-	+
C. lactoaceticus DSM9545	+	-	-	+	+	+	+	-	+
C. kronotskyensis DSM12137	+	-	-	+	+	+	-	-	-

ISSN: 2731-3654