Conversion of fatty aldehydes into alk (a/e)nes by in vitroreconstituted cyanobacterial aldehyde-deformylating oxygenase with the cognate electron transfer system

Table 2 Comparison of the effects of different reducing systems on ADO activity

The reaction mixtures contained 20 μM ADO 1593, 80 μM ferrous ammonium sulfate, 150 μM n-hexadecanal, 75 μM PMS and 750 μM NADH, or 10.26 μM Fd from spinach or PCC7942 and 1.8 μM FNR from spinach or PCC7942 and 2 mM NADPH, in 500 μL of 100 mM HEPES (pH 7.2) containing 0.1 M KCl and 10% glycerol. The reactions were incubated at room temperature for 1 hr at 200 rpm, and the products were extracted with 500 μL ethyl acetate for GC-MS analysis.

ISSN: 2731-3654