Figure 3

The seven NPPs identified as being required for growth on AVICEL. The dry weight of the NPPs when grown in MM plus 2% glucose (A) and the total protein content of the NPPs when grown directly on MM plus AVICEL as a sole carbon source (B). The parental (WT) and NPPs mutant strains were grown in MM plus 1% fructose overnight and then transferred to AVICEL as a sole carbon source for an additional 5 days. The endocellulase activity of the AVICEL culture (C) and the relative expression of two endoglucanase genes, eglA and eglB, in the mycelia isolated from the same cultures (D).