Identification of xylan glucuronoxylan O-methyltransferases (GXM) by DASH. Stem cell wall xylan of Wild type (WT) and gxm mutants was hydrolysed with Np Xyn11A. (A) Aligned electropherograms of Np Xyn11A digests of WT and gxm1 AIR show altered proportions of methylated and unmethylated GlcAX4. (B) Proportion of MeGlcA substitution of xylan was quantified by integration of peak area and analysed using DASHboard. Data are the average of three biological replicates, and are shown +/- s.d. Values significantly different from WT are marked with a * (Student’s t-test, p < 0.05).