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Table 3 Impact of the operation parameters on the inhibitor and sugar level of the dry pretreatment in the helically agitated reactor

From: Helically agitated mixing in dry dilute acid pretreatment enhances the bioconversion of corn stover into ethanol

Pretreatment conditions

Inhibitors in the pretreated CS (g/100 g DM)

Sugars in the pretreated CS (g/100 g DM)

Furfural

5-HMF

Acetic acid

Glucose

Xylose

O-Glu

O-Xyl

Changing temperature

       

165°C, 2.5%, 3 minutes, 50 rpm

0.13 ± 0.02

0.03 ± 0.00

0.45 ± 0.15

0.43 ± 0.01

7.58 ± 0.14

0.94 ± 0.06

9.28 ± 1.11

175°C, 2.5%, 3 minutes, 50 rpm

0.24 ± 0.02

0.06 ± 0.00

0.55 ± 0.01

0.60 ± 0.01

10.05 ± 0.21

0.78 ± 0.03

6.42 ± 0.09

185°C, 2.5%, 3 minutes, 50 rpm

0.63 ± 0.01

0.17 ± 0.01

0.81 ± 0.15

1.01 ± 0.02

10.20 ± 0.02

1.10 ± 0.44

2.84 ± 0.45

Changing acid concentration

       

185°C, 1.5%, 3 minutes, 50 rpm

0.08 ± 0.01

0.04 ± 0.02

0.59 ± 0.06

0.45 ± 0.05

2.39 ± 0.00

0.72 ± 0.04

11.02 ±0.02

185°C, 2.0%, 3 minutes, 50 rpm

0.18 ± 0.06

0.09 ± 0.07

0.58 ± 0.02

0.48 ± 0.00

5.34 ± 0.01

0.77 ± 0.00

8.45 ± 0.38

185°C, 2.5%, 3 minutes, 50 rpm

0.63 ± 0.01

0.17 ± 0.01

0.81 ± 0.15

1.01 ± 0.02

10.20 ± 0.02

1.10 ± 0.44

2.84 ± 0.45

185°C, 3.0%, 3 minutes, 50 rpm

0.89 ± 0.03

0.30 ± 0.03

1.29 ± 0.03

2.41 ± 0.02

9.99 ± 0.11

0.39 ± 0.01

0.81 ± 0.15

185°C, 3.5%, 3 minutes, 50 rpm

0.78 ± 0.02

0.36 ± 0.07

1.24 ± 0.05

2.07 ± 0.02

7.85 ± 0.08

0.45 ± 0.01

0.80 ± 0.10

185°C, 4.0%, 3 minutes, 50 rpm

0.84 ± 0.07

0.41 ± 0.17

1.22 ± 0.16

3.50 ± 0.02

8.34 ± 0.04

0.43 ± 0.07

0.45 ± 0.13

Changing residue time

       

185°C, 2.5%, 1 minute, 50 rpm

0.39 ± 0.03

0.12 ± 0.01

0.73 ± 0.04

0.79 ± 0.00

9.89 ± 0.03

0.90 ± 0.08

2.56 ± 0.35

185°C, 2.5%, 3 minutes, 50 rpm

0.63 ± 0.01

0.17 ± 0.01

0.81 ± 0.15

1.01 ± 0.02

10.20 ± 0.02

1.10 ± 0.44

2.84 ± 0.45

185°C, 2.5%, 5 minutes, 50 rpm

0.80 ± 0.15

0.27 ± 0.03

1.09 ± 0.07

1.21 ± 0.02

7.91 ± 0.06

0.96 ± 0.11

1.35 ± 0.04

185°C, 2.5%, 10 minutes, 50 rpm

0.99 ± 0.10

0.30 ± 0.02

1.13 ± 0.07

1.17 ± 0.01

6.27 ± 0.07

0.69 ± 0.04

0.37 ± 0.08

Changing agitation rate

       

185°C, 2.5%, 3 minutes, 10 rpm

0.59 ± 0.04

0.18 ± 0.02

0.80 ± 0.10

0.99 ± 0.20

10.65 ± 0.35

0.49 ± 0.07

1.53 ± 0.00

185°C, 2.5%, 3 minutes, 30 rpm

0.52 ± 0.02

0.19 ± 0.00

0.79 ± 0.11

0.97 ± 0.27

10.58 ± 0.23

0.78 ± 0.05

2.04 ± 0.04

185°C, 2.5%, 3 minutes, 50 rpm

0.63 ± 0.01

0.17 ± 0.01

0.81 ± 0.15

1.01 ± 0.02

10.20 ± 0.02

1.10 ± 0.44

2.84 ± 0.45

  1. The data in the pretreatment conditions column indicate the pretreatment temperature, acid concentration, and residual time, respectively. All the pretreatment experiments were carried out at 50 rpm. All the experiments were carried out in duplicate. Error was calculated as standard deviation. 5-HMF, 5-hydroxymethylfurfural; CS, corn stover; DM, dry matter; O-Glu, glucan oligomer; O-Xyl, xylan oligomer.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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