Enhanced glycosyl hydrolase production in Aspergillus nidulans using transcription factor engineering approaches

Table 2 List of primers used in this study

Name	Sequence (5'-3')^a
XAKpn	tcttgagctcggtacc TTGACTGTACAATCATG
XAXhoII	ctcag *gatcc* AGGATGACCACAACAT TGTGCTGATCCTG
XBKpn	tcttgagctcggtacc AATTCGAAAAGGTAG
XBXhoII	ctcag *gatcc* AGGATGACCACAACAT GTTGCCGGTTGTTG
RhaATG	AACCAGCTGTGGTCATCCTGGATC
Rha381	aacaaagctt CCTGGTCCATGGCC
PacC923dir	CTGTGGCTGTTGCTAGTGGTCC
PacC1650rev	CCGAGTTACATCATCCTCGCCC
GpdAXho	CCTTCAGTGGACTCGAGTACC
XlnREco	ccgaattc TATACAATGCGC

^aBold nucleotides are restriction sites added. Nucleotides in italics are those coding for the seven first amino acids of RhaA. Capital letters indicate the nucleotides complementary to the genes.

ISSN: 2731-3654